Optimal mixture design of preparation of cold-adapted multiple species inoculant for biomass waste degradation
LUO Lijin1,2,3, WAN Li2, CHEN Hong1, XU Fule3, JIA Wei1, NIE Yilei1, WEN Cuilian4
1. Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou 350007, China;
2. State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China;
3. Fujian Chaoda Modern Agriculture Technology Research Institute, Fuzhou 350003, China;
4. College of Materials Science and Engineering, Fuzhou University, Fuzhou 350108, China
Abstract：In this paper, an optimum mixture design is used for the preparation of cold-adapted multiple species inoculant. Based on the Design Expert software, a quadratic model is established as a function of the component fractions of species, such as the Saccharomyces cerevisiae, the Trichoderma viride and the flora A25-3, on the enzyme activity of the CMCase, the FPase, the cellobiohydrolase, the proteinase and the α-amylase. The response values satisfying all expectations are optimized,and the most excellent combinations of the Saccharomyces cerevisiae, the Trichoderma viride and the flora A25-3 are 0%, 37.24% and 67.76%, respectively. The result of the verification experiment on the formulation is consistent with the prediction. The straw degradation experiments show that the multiple species inoculant has a high level of degradation efficiency for the straw at a low temperature. It suggests that there is a great application prospect of the multiple species inoculant under cold conditions.
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2015, Vol. 33 
