Abstract：Direct atomic force microscopy (AFM) observation and in vitro transcription techniques were used to investigate that mouse cardiac nuclear genes occur on transcription platform of junk DNA. During in vitro transcription, n (n=3 or 4) active gene knots and n-1 gene intervals are put in special permutation and combination to form n (n=3 or 4) different sizes of gene lineage. Different gene knots simultaneously transcribe n mRNA (n=9 or 12) chain complexes, n mRNA (n=9 or 12) chain complexes are respectively linked with two ends of adaptors of related single chain DNAs. If the number of n mRNA (n=9 or 12) decreased, n mRNA (n=9 or 12) may be dissociated from gene lineages and form n mRNA (n=9 or 12) chain complexes through post-transcription modification. These complexes mainly occur in nuclei and are translated into proteins on translation platform, then these proteins are modified to form active proteins. This work shows that direct AFM observation can be used to investigate gene transc...更多ription and regulation, formation of gene lineage and junk DNA interaction.