向结肠癌SW480细胞中导入针对Survivin基因的siRNA，研究RNA干扰对SW480细胞增殖和凋亡的影响，为结肠癌的基因治疗提供实验依据，同时寻求新的、有效的RNA干扰片段。设计3条特异性干扰靶向Survivin基因的siRNA，转染24，48，72h后，Western蛋白印迹法检测Survivin蛋白变化，同时噻唑蓝（MTT）法检测细胞增殖。3条siRNA分别作用于SW480细胞，转染24，48，72h后，3组Survivin蛋白表达均出现下调（P<0.05），在48h时达到顶峰，抑制率分别为66.5%±2.1%，49.6%±2.8%和47.8%±3.1%，差异具有统计学意义（P<0.05）；MTT法显示Survivin基因沉默后，细胞增殖受到明显抑制。脂质体所介导的体外Survivin siRNA，能有效地沉默结肠癌细胞的Survivin基因，从而抑制结肠癌细胞的增殖和促进细胞的凋亡，特别是针对保守区域设计的靶向siRNA，干扰效果更为显著。
Survivin siRNA is transfected to colon cancer cells SW480 and the interference on proliferation and apoptosis is studied to provide the experimental basis for gene therapy of colon cancer and seek new, effective RNA interference fragment. Three siRNA targeting Survivin gene were constructed and transfected into SW480. After 24, 48, and 72h, the change of survivin protein was detected by using Western blot, in the same time, cell proliferation was detected by using MTT assay. The results show that after three siRNA effect on SW480 cells at 24, 48, and 72h, three groups of Survivin protein expression are all appeared the decrement (P<0.05). The expression of Survivin protein reached apex at 48h and the inhibitory rates were 66.5%±2.1%, 49.6%±2.8% and 47.8%±3.1%, respectively, compared with the control group (P<0.05). MTT assay show that the cell proliferation was inhibited after Survivin gene silencing. Liposome-mediated Survivin siRNA can effectively silence the Survivin gene in colon cancer cells, therefore inhibit cancer cell proliferation and promote apoptosis, it is especially designed for targeting conservative regions of siRNA, and interference effects are more significant.
