Abstract: A high-sensitivity piezoelectric quartz crystal immunosensor based on silver enhancement and immuno-colloidal gold technique was developed. Using the sandwich method, the Schistosoma japonicum antigen was fixed in the electrode surface for capturing the Schistosoma japonicum antibody, which then was conjugated with the secondary antibody of goat anti-rabbit IgG labeled marked colloidal gold. Through the reaction catalyzed by colloidal gold upper silver ions reduction, a large number of silver deposited on the nano-around gold to enhance the sensitivity of piezoelectric quartz crystal immunosensor. The results show that the piezoelectric quartz crystal immunosensor based on colloidal gold labeling and silver enhancement is simple to operate, with high sensitivity, wide linear range, and low detection limit. The method has been applied to the rabbit serum antibodies in the determination of Schistosoma japonicum, and satisfactory results have been achieved.
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Received: 23 November 2009
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