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Effects of Heating on Vascular Smooth Muscle Cells |
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Abstract: The aim of this study is to provide some basic research data for magnetic stent hyperthermia for restenosis prevention and treatment after PTCA induced heat of the 316L stainless steel stent with the Alternating Magnetic Field (AMF). The effects of heating on the proliferation, migration, apoptosis and cell cycle of rabbit vascular smooth muscle cells are also studied. VSMCs were incubated at 43℃, 47℃ or 37℃ (control) in temperature-controlled water baths. The cell viability, apoptosis rate, cell cycle rate, cell migration and expression of PCNA protein were measured by MTT assay, flow cytometry, scratch assay and immunocytochemistry, respectively, after the therapy. Results show that heating of coronary stent could be induced by the alternating magnetic field. The proliferation of VSMCs was significantly inhibited by hyperthermia. The cell viability of the 43℃ group was (83.23±2.87)%, while that of the 47℃ group was only (37.58±0.78)%. The apoptosis rate of the 47℃ group(87.37±2.95)% was significantly higher than that of the control group, while that of the 43℃ group saw no statistically significant difference compared with that of the control group. The cell cycle was arrested in S phase. After hyperthermia, the cell migration of the 47℃ group was significantly suppressed, but it had no influence on the cells of the 43℃ group. The expression of PCNA was decreased with the temperature of the bath. All above results suggest that the coronary stents can be heated by the alternating magnetic field. Hyperthermia can depress cell viability and induce VSMCs cell apoptosis. Also it can arrest the cell cycle in S phase and suppress the VSMCs cell migration. The expression of PCNA was reduced by hyperthermia, which may serve as the mechanism of the inhibition of hyperthermia on the proliferation and migration of VSMCs, and the promotion of VSMCs apoptosis.
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Received: 08 April 2010
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