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Preparation of Carboxyl Magnetic Fluid and Surface Modification by Bovine Serum Albumin |
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Abstract: Magnetic nanoparticles are now used in magnetic-mediated hyperthermia for tumor treatment. Magnetic Fe3O4 nanoparticles with a mean diameter of 10~15nm, in the range of superparamagnetism, are normally prepared by the traditional method of co-precipitation from ferrous and ferric electrolyte solutions. This work presents a novel and efficient way to prepare a stable aqueous nanomagnetite dispersion. A stable ferrofluid containing Fe3O4 nanoparticles coated by carboxyl groups was synthesized via improved co-precipitation in the presence of polyacrylic acid. Furthermore, Bovine Serum Albumin (BSA) used as the model targeting molecule was conjugated with magnetic nanoparticles via activation by N-ethyl-N'-(3-dimethylamino-propyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide(NHS). The results indicate that the average diameter of PAA oligomers coated Fe3O4 particles was about 10nm, and had a good dispersion in aqueous phase. In addition, both quantitative analysis and fluorescent microscopy observation confirmed that BSA can be stably conjugated with carboxyl-Fe3O4 particles via EDC and NHS activation.
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Received: 14 April 2010
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