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Morphological Study on the Low Oncogenesis B16 Cell Clone Mutagenized by Space Environment |
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Abstract: The morphological characters of the low oncogenesis melanoma B16 cell clone mutagenized by space environment are studied. The mutated cell clones and control B16 cells were detected with immunofluorescence cytochemistry and confocal lasers scanning microscopy. The mutated cell clones and the control cell clones were imaged by AFM, and the differences between their images were observed. The fluorescent intensity of microfilaments increases in 8# cells. The fiber of cytoskeleton in 8# cells is enlarged. The AFM images show that the shape of the fibronectin secreted by 8# cell clone is different from that of the control clone, the fiber is flatter than that of the control clone, it is bent and circuity; while the fiber of the control clone is like mountain ridge radiated from the cell pseudopodia. There is a few “well” or “hole” like structures on the surface of the 8# cell clone, but there is no such structures observed in the control clone. The surface structure and the distribution of cell cycle are different from those of the control clone. Whether the space environment could increase the immunogenicity of the tumor cells remains to be studied. The findings suggest that there are mutagenesis factors which could mutate the culture tumor cells in the outer space.
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Received: 11 March 2009
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