As compared with the water circumfluence method, a simple and rapid method was developed for the determination of contents of rutin and quercetin in onion by Automated Pressurized Liquid Extractor (APLE). The contents of rutin and quercetin were first determined by High Performance Liquid Chromatography (HPLC) in 368nm and analyzed on the C18 column by a isocratic elution, using a mixture of methanol-phosphoric acid solution(58:42, V/V) as the mobile phase with a flow rate of 1.0mL/min. The method is calibrated by an external standard method. Under the optimal conditions, the correlation coefficient of rutin and quercetin in the concentration range of 0.1~200mg/L is more than 0.999. The average recovery rates of rutin and quercetin in the spiked range of 2.0~100mg/kg are 83.5%~94.2% and 84.8%~91.2%, respectively. And the relative standard deviations are 2.9%~7.4% and 4.2%~6.9%, respectively (n=6). Using the automated pressurized liquid extractor method, the detection limit (LOD) of rutin and quercetin are 0.05mg/kg and 0.03mg/kg, respectively, and the quantitative limits (LOQ) of rutin and quercetin are 0.2mg/kg and 0.1mg/kg, respectively. Therefore, the method developed for the determination of rutin and quercetin in onion by automated pressurized liquid extractor and high performance liquid chromatography gives a good linearity, repeatability and high sensitivity, and is suitable for the rapid determination of rutin and quercetin in vegetables and fruits.
Determination of Contents of Rutin and Quercetin in Onion by Automated Pressurized Liquid Extractor and High Performance Liquid Chromatography[J]. Science & Technology Review, 2011, 29(11-01): 55-58 https://doi.org/10.3981/j.issn.1000-7857.2011.01.007
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