采用静态因素选择实验与动态模拟实验相结合的方法,证实聚乙烯醇-海藻酸钠(PVA-SA)复合凝胶固定的微生物在氯苯污染地下水中对污染物的降解效果优于游离微生物和土著微生物,并且证明了含水层介质粒径与小球粒径比值越大,越有利于固定化微生物对氯苯的迁移的观点,含水层介质粒径与小球粒径比在2—5之间时,15d后氯苯降解率高达78.16%;小球粒径越小,越有利于降解效率的提高。同时得到最佳氯苯降解效果的PVA-SA复合凝胶固定化条件为：PVA浓度80g/L,CaCl₂浓度10g/L,包埋剂与菌液体积比30:1,SA质量分数1.0%。

Chlorobenzene is a synthetic organic compound. It is widely used in the industrial and agricultural productions. It is difficult to be degraded by indigenous microorganisms in the groundwater environment. Immobilization microbial technology is widely used in the water treatment in recent years. Microorganism is immobilized in polymeric gel. It forms an isolated environment for the microorganism. The activity of microorganism and the efficiency of pollutants degradation are improved. The effect of bioaugmentation is enhanced. By using static factors-choosing experiment and dynamic simulation experiment, it is confirmed that PVA-SA composite gel fixed microbial degrade chlorobenzene-contaminated groundwater is more effective than free microorganisms and indigenous microorganisms do. And it is concluded that the bigger ratio of the aquifer medium size to gel size, the more conducive to PVA-SA composite gel migration in groundwater is. It is also concluded that the smaller the gel size is, the higher chlorobenzene degradation efficiency is. For the best degradation effect, PVA-SA composite gel immobilized conditions are PVA concentration of 80g/L, CaCl₂ concentration of 10g/L, the ratio of embedding medium to the broth volume is 30:1, and SA mass fraction is 1.0%.