Comparison of GP73 Glycosylation in Human Hepatocellur Carcinoma Cell Lines with Different Metastatic Potential
ZHANG Shu1, JIANG Kai2, MAO Xiaoyi3, LIU Yinkun1,2
1. Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education; Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China;
2. Cancer Research Center of Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China;
3. School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
Abstract:In this study, Golgi protein 73 (GP73) of hepatocelluler carcinoma (HCC) cell lines with different metastatic potential was purified by antibody affinity chromatography and the purification result was confirmed by LC-MS/MS, providing more proof for GP73 as a biomarker for HCC. Purified GP73 was then analyzed using western blot and lectin blot to obtain its glycosylation level in HCC cell lines with different metastatic potential. We found that glycan of GP73 can be recognized by ConA, AAL, LCA and PHA-E lectins, while the LCA and PHA-E-reactive fraction of GP73 was increased along with the increasing metastatic potential of HCC cell lines. The glycan level of GP73 was different in HCC cell lines with different metastatic potential, and the content of fucose (recognized by LCA) and bisecting structure (recognized by PHA-E) increased in agreement with the metastatic potential.
张舒, 江凯, 茅晓意, 刘银坤. 不同转移潜能人肝癌细胞系中GP73糖基化修饰水平比较[J]. 科技导报, 2014, 32(16): 15-19.
ZHANG Shu, JIANG Kai, MAO Xiaoyi, LIU Yinkun. Comparison of GP73 Glycosylation in Human Hepatocellur Carcinoma Cell Lines with Different Metastatic Potential. journal1, 2014, 32(16): 15-19.
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