Optimization of content determination of monoester-type alkaloids in aconite
ZHANG Chao1, YANG Xinxin2, LIU Ya'nan2, CAI Lu2, WANG Shuai2, PENG Xue2, LI Chaoying2
1. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China;
2. Changchun University of Chinese Medicine, Changchun 130117, China
Abstract:This paper aims to optimize the method for content determination of monoester-type alkaloids in aconite by HPLC in the Chinese Pharmacopoeia (2010 edition). By comparing the methods mentioned in the Chinese Pharmacopoeia and in the literature, we found that there are defects in the extraction method of samples, preparation methods of sample solution, chromatographic conditions, detection methods and determination results in content determination of monoester alkaloids in aconite by HPLC. Therefore, we established a new determination method and compared it with the one stated in the Chinese Pharmacopeia. The established method had better performance than the previous one. This simple method greatly shortened the testing time, reduced the cost, and improved column efficiency, providing a safe, effective, and quality-controllable detection method for aconite.
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