探讨预先电刺激小脑顶核(FNS)对心肌梗死(MI)大鼠心肌炎性细胞因子基因表达的干预作用。分别采用小脑顶核电刺激或小脑顶核毁损(FNL)技术及左冠状动脉前降支(LAD)结扎法建立大鼠实验模型。实验分组:①MI组,单纯结扎LAD;②FNS+MI组,即先FNS,后结扎LAD组;③FNL+FNS+MI组,即毁损小脑顶核后电刺激小脑顶核部位,再行LAD结扎组;④假手术组(Sham组),仅在LAD下穿线不结扎。各组按结扎LAD后的时程又随机分为1、7、21d3个时间点进行研究。应用逆转录聚合酶链反应法检测(RT-PCR)心肌梗死区(IZ)与非梗死区(NIZ)肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、白介素6(IL-6)mRNA的表达,结果发现,MI后TNF-α、IL-β和IL-6mRNA表达不论在IZ还是NIZ均明显增加,其中以冠状动脉结扎后第7d最明显。经FNS预处理后,上述细胞因子在MI后各时间点的表达明显下降;提前毁损小脑顶核后再给予电刺激则无以上作用。研究证实,FNS可下调炎性细胞因子TNF-α、IL-1β、IL-6mRNA的表达。因此,FNS很可能发展成一种使MI患者受益...更多的防治方法。

We investigated the effects of fastigial nucleus electro-stimulation (FNS) in advance on cardiac expression of various proinflamatory cytokines in myocardial infarction (MI) rats. Animal models were created by stimulating fastigial nucleus (FN) with the flow of electricity, using ibotenic acid to produce lesions of FN neurons, ligating the left anterior artery (LAD), respectively. Wistar rats were randomly divided into four groups: group MI was rats with myocardial infarction alone; group FNS+MI was rats receiving FN stimulation for 1 hour before producing MI; group FNL+FNS+MI was rats undergoing the same procedure like group FNS+MI except that there were lesions of the FN five days before producing MI; group Sham was rats without ligating LAD and stimulating FN, just mimicing all the operations. Then every group was randomly divided into three subgroups according to the three time points, that is 1,7,21 days after MI for further study. The mRNA expression levels of tumour necrosis fac...更多tor-alpha(TNF-α), interleukin-1β(IL-1β) and interleukin-6(IL-6) in IZ and NIZ were studied by RT-PCR. Compared to sham group, TNF-α, IL-1βand IL-6 mRNA expression levels increased significantly in IZ or NIZ in MI group, especially on day 7 after ligation. Compared to MI group, the expression levels of TNF-α, IL-1βand IL-6 mRNA in FNS+MI group decreased significantly. There was no decrease effect for the expression of the cytokines when FN neurons were destroyed. We demonstrated that FNS can down-regulate TNF-α, IL-1β, IL-6 mRNA expression. So it can be forecasted that FNS will be beneficial in the prevence and treatment of MI someday.