Abstract：Human kinase domain receptor promoter (KDRp) has been cloned into the pcDNA3 -CDglyTK to construct the pcDNA3-KDRp-CDglyTK specialized expression vector. The pcDNA3-KDRp-CDglyTK plasmid was transfected into ECV304, L9981 and NL9980 cell, and established ECV304-cdtk, L9981-cdtk and NL9980-cdtk cell lines. The above cell lines were treated with 5-FC and/or GCV respectively. The result suggest that cdtk gene was specialized expressed in ECV304-cdtk, L9981-cdtk and NL9980-cdtk KDR-positive cells with KDR gene high expression, and prodrug/KDRp-CDglyTK system was effective in killing effect in KDR-positive cells. The killing effect in combined drug (5-FC and GCV) was remarkable stronger than that in GCV alone and 5-FC alone in the same pre-drug dose, and a significantly synergetic cytotoxic effect was found in vivo and in vitro.
2006, Vol. 24 
