Abstract：The EGCs were proliferated and labeled with 5-bromo-2-deoxyuridine (BrdU), then transplanted into the acute damaged liver by CCL4 through tail vein. 2 and 4 weeks later, the liver was extracted and 10μm-cryostat continuous sections were obtained. The existing and differentiation of the transplanted cells were identified by immunohistochemistry, immunoflorescence double staining and PAS histochemistry for BrdU and hepatic-specific albumin (ALB), and the glycogen. The BrdU positive cells were found in the chronic damaged liver, some cells were BrdU and ALB double positive and PAS positive staining , the structures of damaged liver were also significantly improved. We demonstrated that the transplanted EGCs could be incorporated into the chronic damaged liver and differentiated into hepatocytes in this microenvironment, and its transplantation also could compensate for chronic liver failure. These results suggest that EGCs can be used for potential cellular hepatoplasty to treat damaged l...更多iver.