Abstract:To investigate the effects of recombinant human BMP (rhBMP)-2 and -6 on osteosarcoma in treating human osteosarcoma cell lines MG63 and U2OS with rhBMP-2 and rhBMP-6, the osteosarcoma cells in experimental groups were treated with rhBMPs-CMs, those in control groups with rGFP-CM, and those in blank groups with neither. Cell proliferation, apoptosis transmigration and differentiation were detected at different times by Trypan blue exclusion test, transwell-room, terminal deoynucleotidyl transferase mediated dUTP nick end labeling (TUNEL)/acridine orange-ethidium bromide (AO/EB) double fluorescent dye staining and the alkaline phosphatase (ALP) activity, respectively. The main results are as follows. (1) There are no significant differences of all indexes tested between the control groups and the blank (all with P>0.05). (2) Compared with the control groups and the blank groups, the cell survival rates see a significant decrease when being treated with rhBMP-2 and rhBMP -6 (P<0.01). (3) The apoptosis indexes are increased (P<0.01) and the results from TUNEL and AO/EB method are consistent. (4) The cell numbers of transmembrane are significantly decreased in both MG63 and U2OS (P<0.01). (5) The ALP activity is increased after being treated with rhBMP-2 and rhBMP-6 for 3 days in MG63 and for 5 days in U2OS (P<0.01). It is suggested that rhBMP-2 and -6 could inhibit the proliferation and transmigration of MG63 and U2OS, induce their apoptosis and differentiation.