Mammalian cell cloning is the most compelling high-technology in life sciences in the late 20th century. It is important because of its roles played in the reproduction of good breeding stock, reduction of the number of experimental animals, reserving of animal genetic diversity, saving of endangered animals, transgenic animal breeding and other aspects. A variety of cloning animals have been made successful, but with a low efficiency and frequent abnormality in birth weight and physiological conditions, which are discussed in detail in this paper, together with the current developments of animal cloning, and the technologies involved in cloning animals. The methods to remove the nuclei from oocytes and to reconstruct embryos are highlighted. The application prospects are also commented.
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