Analysis of the Blood Amino Acid Metabonomics of Cervical Intraepithelial Neoplasia and Cervical Cancer by High-performance Liquid Chromatography
HASIM Ayshamgul1, SAIMAITI Aini2, KUERBAN Shajidai1, ZHENG Jianhe1, MA Junqi3
1. College of Basic Medicine, Xinjiang Medical University, Urumqi 830011, China;
2. Department of Infection, Shuimogou Area Hospital, Urumqi 830011, China;
3. Department of Gynecology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830011, China
Abstract：To investigate the amino acids in plasma samples from patients with the cervical intraepithelial neoplasia (CIN) and the cervical cancer and from healthy volunteers, to assess their clinical significance and to have insights into the cervical cancer amino acid metabolism and the systemic effects, 26 plasma samples from the CIN patients, 22 plasma samples from the CSCC patients and 35 samples from healthy volunteers are analyzed by the HPLC, and the partial least-squares discriminant analysis (PLS-DA) is employed to perform the pattern recognition analysis of the HPLC spectra. It is shown that there is a significant difference in plasma amino acids between those from patients with the CIN and cervical cancers and from healthy subjects. Compared to the healthy controls, the plasma aspartate, the glutamate, the asparagine, the serine, the glycine, the histidine, the taurine, the alanine, the proline, the tyrosine, the valine, the methionine, the lysine, the isoleucine, the leucine, and the phenylalanine decrease gradually from the CIN to cervical cancers with statistical significance (P<0.05), however the arginine and the threonine decrease dominantly in the CIN plasma than those in the CSCC. These findings indicate that the tumor cells may uptake a specific amino acid selectively from the plasma to meet their needs at different stages of growth.
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2014, Vol. 32 
