Abstract:To determine the HPLC fingerprint of the ultramicro rhizoma Gastrodiae and to test its potent constituents and content. Chromatographic column: Hypersil BDS C18(4.6 mm×200 mm,5μm). Mobile phase: 0.05% phosphoric acid in water. Column temperature: 25℃. Flow rate: 1.0 ml·min-1. Wavelength: 220nm. The fingerprint of ultramicro rhizoma Gastrodiae was established and 7common peaks are in the fingerprint. Conclusion is that methods and data can be used to control the quality of the ultramicro rhizoma Gastrodiae.