Effect of Novel TiO2 Nanotube Dialysis and Traditional Dialysis Films on Cells Growth State and Their Comparison
ZHU Wen1,2, LI Jiwei1,2, LIU Jianfeng3, TAN Cong1, CHONG Baohe1, YANG Xuebing1
1. State Key Laboratory of Material Processing and Die & Mould Technology, Huazhong University of Science and Technology, Wuhan 430074, China;2. State Key Laboratory of Functional Ceramics, Shenzhen 518057, Guangdong Province, China;3. School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
Abstract:Novel high intensity TiO2 nanotube array film was fabricated via electrochemical anodization, and then open-ended TiO2 nanotubes array membrane was obtained by HF gas etching the bottom of nanotubes. HK-2 cells and HUVEC cells were cultivated on the open-ended TiO2 nanotube array surface and polymer dialysis film surface, and biomembrane materials which have physiological function are successfully achieved. Cell viability of TiO2 nanotube array, Polyether Sulfone (PES), mixed cellulose ester, and regenerated cellulose was investigated by using MTT method. Cell morphology on these membranes was examined by using fluorescence microscope and Filed Emission Scanning Electron Microscope (FESEM). The results indicate that TiO2 nanotube array film is of the most benefit to cell adhesion and cell proliferation, and the cell viability is the highest, and the second place belongs to the PES membrane, the worst case is regenerated cellulose that is unsuitable for cell proliferation and cell adhesion. Fluorescence microscope shows that TiO2 nanotube array membrane could promote the cell adhesion and cell proliferation batter than polymeric membrane does. It demonstrates that TiO2 nanotube array film has excellent biocompatibility, overcoming the deficiency of traditional dialysis materials, improving the cell adhesive; and the film is the ideal candidate materials for biological dialysis.
朱文;李继伟;刘剑峰;谭聪;崇保和;杨学兵. 新型TiO2纳米管透析膜与传统透析膜对细胞生长状态的影响[J]. , 2013, 31(18): 15-21.
ZHU Wen;LI Jiwei;LIU Jianfeng;TAN Cong;CHONG Baohe;YANG Xuebing. Effect of Novel TiO2 Nanotube Dialysis and Traditional Dialysis Films on Cells Growth State and Their Comparison. , 2013, 31(18): 15-21.