为探讨信号转导通路与异常黑胆质证神经-内分泌-免疫(NEI)网络功能紊乱的关系,采用基因芯片技术检测异常黑胆质证与非异常黑胆质(异常血液质、异常黏液质、异常胆液质)证白细胞结构基因表达水平,筛选差异表达基因,利用生物信息学技术分析差异表达基因参与的相关信号转导通路。芯片结果提示,与非异常黑胆质证相比,异常黑胆质证白细胞中有75个结构基因表达上调,生物信息学分析显示差异表达基因中富集到信号转导生物学过程的基因有12个,这些基因主要涉及MAPK、Toll样受体和Wnt信号转导通路等。由此可见,MAPK、Toll样受体、Wnt信号转导通路在异常黑胆质证患者体内存在异常激活现象,这可能与异常黑胆质证神经-内分泌-免疫网络功能紊乱密切相关。

In order to study the relationship between the signal transduction pathway and the disorder of the neuro-endocrine-immunity network in abnormal Savda Hilit, the blood samples of the abnormal Savda Hilit group and the non-abnormal Savda Hilit group (abnormal Kan Hilit, abnormal Belhem Hilit, abnormal Safra Hilit) were collected. After separating the white blood cell and extracting the RNA, the Affymetrix gene expression microarray technology was applied to detect the gene expression of the two groups. Then the differentially expressed genes were selected and a bioinformatic analysis was made by using the CapitalBio Molecule Annotation System (MAS) to find the genes related to the signal transduction pathway. The result of chip shows that 75 genes are up-regulated in the abnormal Savda Hilit group comparing with the non-abnormal Savda Hilit group. The bioinformatic analysis shows that 12 genes are concentrated in the signal transduction pathways of the biological process, involved in the MAPK, Toll-like receptor, the Wnt signal transduction pathway. These findings indicate that the abnormal activation of the MAPK, Toll-like receptor, the Wnt signal transduction pathway may be related to the disorder of the neuro-endocrine-immunity network in the abnormal Savda Hilit group.