丛枝菌根对采煤沉陷区紫穗槐生长及土壤改良的影响

doi:10.3981/j.issn.1000-7857.2014.11.003

摘要
图/表
参考文献
相关文章 (5)

全文: PDF (889 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要丛枝菌根技术是目前矿区生态修复的重要手段之一。通过向紫穗槐接种丛枝菌根（AM），研究接种后2~14 个月间菌根对紫穗槐生长和土壤改良的影响。结果表明，与不接种相比，接种AM 极显著提高植物成活率7.2%~9.7%，株高极显著增加34%~62%、冠幅极显著增加39%~65%；极显著提高菌根侵染率16%~21%，极显著提高菌丝密度50%~70%；菌根侵染率、菌丝密度与土壤有机碳、全氮、速效磷、速效钾、碱解氮含量显著或极显著正相关；接种AM 能够显著降低土壤pH 值，提高土壤有机碳、全氮、速效磷、速效钾、碱解氮含量；球囊霉素相关土壤蛋白是土壤有机质的重要组成部分，可以灵敏反映土壤质量的变化。接种AM能够促进采煤沉陷区紫穗槐的生长发育和土壤改良。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	王瑾
	毕银丽
	邓穆彪
	邹慧
	孙江涛
	解文武

关键词 ：丛枝菌根, 土壤质量, 侵染率, 球囊霉素相关土壤蛋白

Abstract：Arbuscular mycorrhizal technology is one of the important means for ecological restoration in mining areas. In this paper, effects of the arbuscular mycorrhizal (AM) inoculation on the growth and development of Amorpha Fruticosa L. and soil quality were studied between 2 and 14 months post-inoculation. The results showed that compared with that of the non-inoculated Amorpha Fruticosa L., the survival rate of inoculated one increased by 7.2%-9.7%, the plant height markably increased by 34%-62%, the crown diameter significantly increased by 39%-65%, the mycorrhizal infection rate significantly increased by 16%-21%, and the hyphal density increased by 50%-70%. The mycorrhizal infection rate and hyphal density had significantly or highly significantly positive correlations with contents of organic carbon, total nitrogen, available phosphorus and potassium, alkali-hydrolyzable nitrogen in the soil. The soil pH significantly decreased, and the contents of soil organic carbon, total nitrogen, available phosphorus and potassium, alkali-hydrolyzable nitrogen significantly increased by the inoculation of mycorrhiza. Glomalin related soil protein is an important composition of organic matter in soil, which can reflect small changes of soil quality. These results show that AM can promote growth and development of Amorpha Fruticosa L. and soil improvement in coal mining subsidence areas.

Key words： arbuscular mycorrhiza soil quality mycorrhizal infection rate glomalin related soil protein

收稿日期: 2014-01-13

ZTFLH:

S154

基金资助:“十二五”国家科技支撑计划项目（2012BAC10B03）

通讯作者: 毕银丽，教授，研究方向为土地复垦及微生物复垦技术，电子信箱：ylbi88@163.com E-mail: ylbi88@163.com

作者简介: 王瑾，博士研究生，研究方向为土地复垦与生态重建，电子信箱：swangj2005@126.com

引用本文:

王瑾, 毕银丽, 邓穆彪, 邹慧, 孙江涛, 解文武. 丛枝菌根对采煤沉陷区紫穗槐生长及土壤改良的影响[J]. 科技导报, 2014, 32(11): 26-32.
WANG Jin, BI Yinli, DENG Mubiao, ZOU Hui, SUN Jiangtao, XIE Wenwu. Effects of Arbuscular Mycorrhiza on Growth of Amorpha Fruticosa L. and Soil Improvement in Coal Mining Subsidence Area. journal1, 2014, 32(11): 26-32.

链接本文:

http://www.kjdb.org/CN/10.3981/j.issn.1000-7857.2014.11.003 或 http://www.kjdb.org/CN/Y2014/V32/I11/26

[1] 李建华, 郜春花, 卢朝东, 等. 菌剂与肥料配施对矿区复垦土壤白三叶草生长的影响[J]. 中国生态农业学报, 2011, 19(2): 280-284. Li Jianhua, Gao Chunhua, Lu Chaodong, et al. Effect of combined application of microbial inoculum and fertilizeron white clover growth in reclaimed mine soil[J]. Chinese Journal of Eco-Agriculture, 2011, 19 (2): 280-284.
[2] 胡振琪, 魏忠义, 秦萍. 矿山复垦土壤重构的概念与方法[J]. 土壤, 2005, 37(1): 8-12. Hu Zhenqi, Wei Zhongyi, Qin Ping. Concept and methods for soil reconstruction in mined land reclamation[J]. Soils, 2005, 37(1): 8-12.
[3] 崔树军, 谷立坤, 廉有轩, 等. 煤矿废弃地的微生物修复技术[J]. 金属矿山, 2010(4): 176-179. Cui Shujun, Gu Likun, Lian Youxuan, et al. Research of microbiology technology in ecological remediation of the abandoned coal mining land[J]. Metal Mine, 2010(4): 176-179.
[4] 张桃林, 潘剑君, 赵其国. 土壤质量研究进展与方向[J]. 土壤, 1999, 31(1): 2-8. Zhang Taolin, Pan Jianjun, Zhao Qiguo. Research progress and direction of soil quality[J]. Soils, 1999, 31(1): 2-8.
[5] 岳辉, 毕银丽, Y. Zhakypbek, 等. 接种菌根对神东矿区采煤沉陷地的生态修复效应[J]. 科技导报, 2012, 30(36): 56-60. Yue Hui, Bi Yinli, Zhakypbek Y, et al. Ecological reclamation effect of arbuscualr mycorrhizal inoculum on subsided land in the area of shendong coal mine[J]. Science & Technology Review, 2012, 30(36): 56-60.
[6] Smith S E, Read D J. Mycorrhizal symbiosis[M]. London: Academic Press, 1997.
[7] Juge C, Prévost D, Bertrand A, et al. Growth and biochemical responses of soybean to double and triple microbial associations with Bradyrhizobium, Azospirillum and arbuscular mycorrhizae[J]. Applied Soil Ecology, 2012, 61(10): 147-157.
[8] 弓明钦, 陈应龙, 仲崇禄. 菌根研究及其应用[M]. 北京: 中国林业出版社, 1997. Gong Mingqin, Chen Yinglong, Zhong Chonglu. Applying research of mycorrhiza[M]. Beijing: China Foresrty Press, 1997.
[9] Vergeer P, Berg L J L, Baar J, et al. The effect of turf cutting on plant and arbuscular mycorrhizal spore recolonisation: Implications for heathland restoration[J]. Biological Conservation, 2006, 129(2): 226- 235.
[10] Wright S F, Upadhyaya A. A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi[J]. Plant and Soil, 1998, 198(1): 97-107.
[11] 鲍士旦. 土壤农化分析[M]. 北京: 中国农业出版社, 2005. Bao Shidan. Agricultural chemical soil analysis[M]. Beijing: China Agriculture Press, 2005.
[12] Abbott L K, Robson A D, De Boer G. The effect of phosphorus on the formation of hyphae in soil by the vesicular- arbuscular mycorrhizal fungus, Glomus Fasciculatum[J]. New Phytologist, 1984, 97(3): 437- 446.
[13] Phillips J M, Hayman D S. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection[J]. Transactions of the British Mycological Society, 1970, 55(1): 158-160.
[14] 杜善周, 毕银丽, 吴王燕, 等. 丛枝菌根对矿区环境修复的生态效应[J]. 农业工程学报, 2008, 24(4): 113-116. Du Shanzhou, Bi Yinli, Wu Wangyan, et al. Ecological effects of arbuscular mycorrhizal fungi on environmental phytoremediation in coal mine areas[J]. Transactions of the CSAE, 2008, 24(4): 113-116.
[15] 杜介方, 张彬, 解宏图, 等. 不同施肥处理对球囊霉素土壤蛋白含量的影响[J]. 土壤通报, 2011, 42(3): 573-577. Du Jiefang, Zhang Bin, Xie Hongtu, et al. The effect of fertilization treatments on the concentration of GRSP[J]. Chinese Journal of Soil Science, 2011, 42(3): 573-577.
[16] Nichols K A, Wright S F. Carbon and nitrogen in operationally defined soil organic matter pools[J]. Biology and Fertility of Soils, 2006, 43(2): 215-220.
[17] Halvorson J J, Gonzalez J M. Tannic acid reduces recovery of watersoluble carbon and nitrogen from soil and affects the composition of Bradford-reactive soil protein[J]. Soil Biology and biochemistry, 2006, 40(1): 186-197.
[18] He X, Li Y, Zhao L. Dynamics of arbuscular mycorrhizal fungi and glomalin in the rhizosphere of Artemisia ordosica Krasch. in Mu Us sandland, China[J]. Soil Biology and Biochemistry, 2010, 42(8): 1313- 1319.
[19] Graham J H, Linderman R G, Menge J A. Development of external hyphae by different isolates of mycorrhizal Glomus spp. in relation to root colonization and growth of troyer citrange[J]. New Phytologist, 1982, 91(2): 183-189.
[20] 符亚儒, 高保山, 封斌, 等. 陕北榆林风沙区防风固沙林体系结构配置与效益研究[J]. 西北林学院学报, 2005, 20(2): 18-23. Fu Yaru, Gao Baoshan, Feng Bin, et al. Structure configuration and protecting benefit of Yulin sandbreak forest system in northern Shaanxi[J]. Journal of Northwest Forestry University, 2005, 20(2): 18-23.
[21] Heidari M, Karami V. Effects of different mycorrhiza species on grain yield, nutrient uptake and oil content of sunflower under water stress[J]. Journal of the Saudi Society of Agricultural Sciences, 2014, 13(1): 9-13.
[22] Marschner H, Dell B. Nutrient uptake in mycorrhizal symbiosis[J]. Plant and Soil, 1994, 159(1): 89-102.
[23] 高子勤, 张淑香. 连作障碍与根际微生态研究Ⅰ.根系分泌物及其生态效应[J]. 应用生态学报, 1998, 9(5): 549-554. Gao Ziqin, Zhang Shuxiang. Continuous cropping obstacle and rizospheric microecology I. Root exudates and their ecological effects[J]. Chinese Journal of Applied Ecology, 1998, 9(5): 549-554.