低温低碳氮比好氧反硝化菌的筛选及鉴定

doi:10.3981/j.issn.1000-7857.2014.11.004

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摘要传统的反硝化工艺存在反硝化细菌的世代时间较长（特别是在低温的冬季）、水力停留时间长、运行和投资费用大等问题。为寻找更好的反硝化细菌，从冬季北方城市污水厂驯化活性污泥中分离出1 株耐低温、低碳氮比，且脱氮率较高的好氧反硝化菌株，命名为HFX08。初步鉴定该菌株为假单胞菌属（Pseudomonas），G^-菌。该菌株的最适生长温度为10~20℃，生长曲线符合S 型曲线，拟合方程相关性系数达到了差异极显著水平。随着碳氮比的增加，该菌株的反硝化速率随之提高，脱氮率最高可达92%。

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关键词 ：好氧反硝化菌, 假单胞菌属, 脱氮率, 碳氮比, 低温

Abstract：The conventional denitrification process has limited applications due to its disadvantages, such as long generation time of denitrifying bacteria, especially in cold winter, long hydraulic retention time and high costs. In this study, an aerobic denitrifying bacterium designated as HFX08 was isolated from a domestic sewage plant in a northern city of China in winter. The strain HFX08 exhibited high denitrification performance under low C/N ratio at low temperature. The results indicated that the strain HFX08 was G^- and identified as Pseudomonas sp. The optimum temperature for its growth is in the range of 10-20℃. Its growth curve was fitted to S growth curve, whose fitting coefficient reached the level of extremely significant difference. The nitrogen removal rate by strain HFX08 increased with increasing C/N ratio, up to 92%.

Key words： aerobic denitrifying bacterium Pseudomonas nitrogen removal rate C/N ratio low temperature

收稿日期: 2013-11-15

ZTFLH:

X172

基金资助:吉林省重大科技攻关项目（20130204054SF）；国家环境保护公益性行业科研专项（201009009）

通讯作者: 张凤君，教授，研究方向为水处理技术，电子信箱：zhangfengjun@jlu.edn.cn E-mail: zhangfengjun@jlu.edn.cn

作者简介: 周兰影，博士研究生，研究方向为水处理技术，电子信箱：735189410@qq.com

引用本文:

周兰影, 马秀兰, 张晨东, 王呈玉, 张凤君. 低温低碳氮比好氧反硝化菌的筛选及鉴定[J]. 科技导报, 2014, 32(11): 33-37.
ZHOU Lanying, MA Xiulan, ZHANG Chendong, WANG Chengyu, ZHANG Fengjun. Screening and Identification of an Aerobic Denitrifying Bacterium with Low C/N Ratio at Low Temperature. journal1, 2014, 32(11): 33-37.

链接本文:

http://www.kjdb.org/CN/10.3981/j.issn.1000-7857.2014.11.004 或 http://www.kjdb.org/CN/Y2014/V32/I11/33

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