Anticancer Effect of Magnetic Targeting Hyperthermia on Mouse Pancreatic Cancer
WANG Lufang1, TANG Jintian2, OUYANG Weiwei3, DONG Jian4, ZHAO Lingyun2, WANG Xiaowen2, HU Bing1
1. Department of Oncology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China;
2. Key Laboratory of Particle & Radiation Imaging of the Ministry of Education; Department of Engineering Physics, Tsinghua University, Beijing 100084, China;
3. Department of Thoracic Oncology, Affiliated Hospital of Guiyang Medical College, Guizhou Cancer Hospital, Guiyang 550004, China;
4. Department of Oncology, The First Affiliated Hospital of Kunming University of Medical Science, Kunming 650032, China
Abstract：This paper aims to assess the therapeutic effect of magnetic fluid hyperthermia (MFH) on mouse pancreatic cancer in vitro and in vivo. In vitro and in vivo experiments were respectively established using the unique mouse pancreatic cancer cell line (MPC- 83). Apoptosis and morphological changes of the MPC-83 were measured with flow cytometry and microscopy after heating to 42, 46, and 50℃ with water bath for 30 min. Four-week-old female Kunming mice were selected to establish subcutaneous pancreatic cancer model. The therapeutic effect (46℃ and 50℃) was evaluated by detecting various influences including pathological examination. Flow cytometry revealed the apoptosis and necrosis rate of MPC-83 were 46.13% (46℃) and 89.33% (50℃). Fourteen days after hyperthermia, the tumor growth rate were -0.64±0.73 and -0.72±0.79, which were significantly lower than that in each control group (P<0.05). Compared with control groups, tumor growth in the experimental group was markedly inhibited. Pathological examination showed that magnetic nanoparticles were distributed between tumor cells, and some of them were ingested by phagocytes. Fourteen days after hyperthermia, there were no subcutaneous nodules with only the MF remaining. In each control group, tumor cells grew very well, and hyperchromatic nuclei and pathological karyokinesis were seen simultaneously. MFH can reach perfect therapeutic temperature and heat tumor tissue specifically, inhibiting the growth of mouse pancreatic cancer and prolonging the survival time of the mice apparently. MFH has satisfactory therapeutic efficacy for mouse pancreatic cancer.
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2014, Vol. 32 
