附子单酯型生物碱含量测定方法的优化

doi:10.3981/j.issn.1000-7857.2016.2.033

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摘要对比《中国药典》与文献中附子单脂型生物碱HPLC 含量测定方法,发现附子HPLC 含量测定中样品的提取方法、供试品溶液的制备方法、色谱条件、检测方法和含量测定结果均欠佳,故建立附子新的含量测定方法并与《中国药典》含量测定方法比较。优化后的附子单脂型生物碱HPLC 含量测定方法优于《中国药典》方法。研究建立的附子HPLC 测定方法简便,可缩短检测时间,降低检测成本,提高了柱效,为附子安全、有效、质量可控提供更科学完善的检测方法。

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	张超
	杨辛欣
	刘亚楠
	蔡璐
	王帅
	彭雪
	李超英

关键词 ：附子, 中国药典, 高效液相色谱法, 单酯型生物碱

Abstract：This paper aims to optimize the method for content determination of monoester-type alkaloids in aconite by HPLC in the Chinese Pharmacopoeia (2010 edition). By comparing the methods mentioned in the Chinese Pharmacopoeia and in the literature, we found that there are defects in the extraction method of samples, preparation methods of sample solution, chromatographic conditions, detection methods and determination results in content determination of monoester alkaloids in aconite by HPLC. Therefore, we established a new determination method and compared it with the one stated in the Chinese Pharmacopeia. The established method had better performance than the previous one. This simple method greatly shortened the testing time, reduced the cost, and improved column efficiency, providing a safe, effective, and quality-controllable detection method for aconite.

Key words： aconite the Chinese Pharmacopeia HPLC monoester alkaloids

收稿日期: 2015-03-01

基金资助:吉林省科技发展计划项目(YYZX201265)

通讯作者: 李超英,教授,研究方向为药物新型递药系统与新药,电子信箱:chaoying_li@126.com E-mail: chaoying_li@126.com

作者简介: 张超,博士研究生,研究方向为肿瘤药理和药物,电子信箱:zhangchao_0213@126.com;杨辛欣(共同第一作者),讲师,研究方向为药物新型递药系统,电子信箱:xinxin_yang1980@126.com

引用本文:

张超, 杨辛欣, 刘亚楠, 蔡璐, 王帅, 彭雪, 李超英. 附子单酯型生物碱含量测定方法的优化[J]. 科技导报, 2016, 34(2): 200-204.
ZHANG Chao, YANG Xinxin, LIU Ya'nan, CAI Lu, WANG Shuai, PENG Xue, LI Chaoying. Optimization of content determination of monoester-type alkaloids in aconite. Science & Technology Review, 2016, 34(2): 200-204.

链接本文:

http://www.kjdb.org/CN/10.3981/j.issn.1000-7857.2016.2.033 或 http://www.kjdb.org/CN/Y2016/V34/I2/200

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