Abstract:To investigate the regulation role of Ad36 on PPARγ and CIDEC in Ad36 induced adipocyte differentiation,human adiposederived mesenchymal stem cells(hAMSC)are infected with adenovirus and the adipocyte induced by Ad36 are identified using RT-qPCR and oil red experiments. The expressions of PPARγ and CIDEC genes are determined by RT-qPCR and Western Blotting in Ad36 induced hAMSC. Using GW9662, the PPARγ inhibitor inhibits the expression of PPARγ, then the CIDEC gene expression is detected by Western Blotting in the Ad36 induced hAMSC. Ad36 induced hAMSC differentiates into human adipocyte in vitro, the glucose content in media is reduced and TG content in cells is increased significantly during the differentiation compared to the control group (P<0.05), and the expressions of PPARγ and CIDEC genes are upregulated gradually compared to the control group (P<0.05). CIDEC protein expression is downregulated after treating Ad36 induced human adipocyte by GW9662, the PPARγ specific inhibitor, compared to Ad36 induced group (P<0.05). In this study it is found that Ad36 upregulates CIDEC gene expression through PPARγ in Ad36 induced human adipocytes.
[1] 张春雨, 王德生, 张国华, 等. 中国内蒙古自治区牧区蒙、汉民族55岁 以上居民超重和肥胖病患病率的流行病学调查[J]. 中华内分泌代谢 杂志, 2014, 30(6): 464-467. Zhang Chunyu, Wang Desheng, Zhang Guohua, et al. Epidemiological investigation on the prevalence of obesity and overweight in Mongolian and Han residents aged over 55 years in the pastoral area of Inner Mongolia, China[J]. Chinese Journal of Endocrinology and Metabolism, 2014, 30(6): 464-467.
[2] Dhurandhar N V, Israel B A, Kolesar J M, et al. Increased adiposity in animals due to a human virus[J]. International Journal of Obesity and Related Metabolic Disorders: Journal of the International Association for the Study of Obesity, 2000, 24(8): 989-996.
[3] Atkinson R L, Dhurandhar N V, Allison D B, et al. Human adenovirus-36 is associated with increased body weight and paradoxical reduction of serum lipids[J]. International Journal of Obesity, 2005, 29(3): 281-286.
[4] Rogers P M, Fusinski K A, Rathod M A, et al. Human adenovirus Ad-36 induces adipogenesis via its E4 orf-1 gene[J]. International Journal of Obesity, 2008, 32(3): 397-406.
[5] Pasarica M, Mashtalir N, Mcallister E J, et al. Adipogenic human adenovirus Ad-36 induces commitment, differentiation, and lipid accumulation in human adipose-derived stem cells[J]. Stem Cells, 2008, 26(4): 969-978.
[6] Wu C, Zhang Y, Sun Z, et al. Molecular evolution of Cide family proteins: novel domain formation in early vertebrates and the subsequent divergence[J]. BMC Evolutionary Biology, 2008, 8: 159-174.
[7] Jiao Y, Mao X, Chang X, et al. Adenovirus36 infection expresses cellular APMI and Visfatin genes in overweight Uygur individuals[J]. Diagnostic Pathology, 2014, 9: 83-89.
[8] 努尔比耶·努尔麦麦提, 焦谊, 伊力亚斯·艾萨, 等. 腺病毒36感染对 人脂肪源性间充质干细胞PPARγ和adiponectin 基因表达的影响[J]. 新 疆医科大学学报, 2015, 38(5): 558-563. Nuerbiye Nuermaimaiti, Jiao Yi, Yiliyasi Aisa, et al. The variation of PPARγ and adiponectin genes expression in adenouirus-36 infected human adipose-derived mesenchymal stem cell[J]. Journal of Xinjiang Medical University, 2015, 38(5): 558-563.
[9] Allison D B, Downey M, Atkinson R L, et al. Obesity as a disease: A white paper on evidence and arguments commissioned by the Council of the Obesity Society[J]. Obesity, 2008, 16(6): 1161-1177.
[10] Yeh W C, Cao Z, Classon M, et al. Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP family of leucine zipper proteins[J]. Genes & Development, 1995, 9(2): 168-181.
[11] Hamm J K, El Jack A K, Pilch P F, et al. Role of PPAR gamma in regulating adipocyte differentiation and insulin-responsive glucose uptake[J]. Annals of the New York Academy of Sciences, 1999, 892 (1): 134-145.
[12] Wu Z, Rosen E D, Brun R, et al. Cross-regulation of C/EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and insulin sensitivity[J]. Molecular Cell, 1999, 3(2): 151-158.
[13] Kawada T. Lipid metabolism related nuclear receptor-The structure, function, expression and classification of peroxisome proliferationactivated receptor (PPAR) [J]. Nihon Rinsho. Japanese Journal of Clinical Medicine, 1998, 56(7): 1722-1728.
[14] Takeyama K, Kodera Y, Suzawa M, et al. Peroxisome proliferatoractivated receptor (PPAR)-structure, function, tissue distribution, gene expression[J]. Nihon Rinsho. Japanese Journal of Clinical Medicine, 2000, 58(2): 357-363.
[15] Fajas L, Fruchart J C, Auwerx J. PPARgamma3 mRNA: A distinct PPARgamma mRNA subtype transcribed from an independent promoter[J]. FEBS Letters, 1998, 438(1-2): 55-60.
[16] Elbrecht A, Chen Y, Cullinan C A, et al. Molecular cloning, expression and characterization of human peroxisome proliferator activated receptors gamma 1 and gamma 2[J]. Biochemical and unications, 1996, 224(2): 431-437.
[17] Ren D, Collingwood T N, Rebar E J, et al. PPARgamma knockdown by engineered transcription factors: Exogenous PPARgamma2 but not PPARgamma1 reactivates adipogenesis [J]. Genes & Development, 2002, 16(1): 27-32.
[18] Li J Z, Li P. Cide proteins and the development of obesity[J]. Novartis Foundation Symposium, 2007, 286(155-159): 196-203.
[19] Boren J, Taskinen M R, Olofsson S O, et al. Ectopic lipid storage and insulin resistance: A harmful relationship[J]. Journal of Internal Medicine, 2013, 274(1): 25-40.
[20] Saraf N, Sharma P K, Mondal S C, et al. Role of PPARg2 transcription factor in thiazolidinedione-induced insulin sensitization[J]. The Journal of Pharmacy and Pharmacology, 2012, 64(2): 161-171.
[21] Seale P, Bjork B, Yang W, et al. PRDM16 controls a brown fat/skeletal muscle switch[J]. Nature, 2008, 454(7207): 961-967.
[22] Puri V, Konda S, Ranjit S, et al. Fat-specific protein 27, a novel lipid droplet protein that enhances triglyceride storage[J]. The Journal of Biological Chemistry, 2007, 282(47): 34213-34218.
[23] Keller P, Petrie J T, De Rose P, et al. Fat-specific protein 27 regulates storage of triacylglycerol[J]. The Journal of Biological Chemistry, 2008,283(21): 14355-14365.
[24] Nian Z, Sun Z, Yu L, et al. Fat-specific protein 27 undergoes ubiquitin-dependent degradation regulated by triacylglycerol synthesis and lipid droplet formation[J]. The Journal of Biological Chemistry, 2010, 285(13): 9604-9615.
[25] Matsusue K, Kusakabe T, Noguchi T, et al. Hepatic steatosis in leptindeficient mice is promoted by the PPARgamma target gene Fsp27 [J]. Cell Metabolism, 2008, 7(4): 302-311.
[26] Kim Y J, Cho S Y, Yun C H, et al. Transcriptional activation of Cidec by PPARgamma2 in adipocyte [J]. Biochemical and Biophysical Rsearch Communications, 2008, 377(1): 297-302.