Abstract：To investigate the regulation role of Ad36 on PPARγ and CIDEC in Ad36 induced adipocyte differentiation,human adiposederived mesenchymal stem cells(hAMSC)are infected with adenovirus and the adipocyte induced by Ad36 are identified using RT-qPCR and oil red experiments. The expressions of PPARγ and CIDEC genes are determined by RT-qPCR and Western Blotting in Ad36 induced hAMSC. Using GW9662, the PPARγ inhibitor inhibits the expression of PPARγ, then the CIDEC gene expression is detected by Western Blotting in the Ad36 induced hAMSC. Ad36 induced hAMSC differentiates into human adipocyte in vitro, the glucose content in media is reduced and TG content in cells is increased significantly during the differentiation compared to the control group (P<0.05), and the expressions of PPARγ and CIDEC genes are upregulated gradually compared to the control group (P<0.05). CIDEC protein expression is downregulated after treating Ad36 induced human adipocyte by GW9662, the PPARγ specific inhibitor, compared to Ad36 induced group (P<0.05). In this study it is found that Ad36 upregulates CIDEC gene expression through PPARγ in Ad36 induced human adipocytes.
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2016, Vol. 34 
