The development of a Melanogrammus aeglefinus DNA standard sample
JIANG Dan1, LIU Yu2, DING Jian1, MA Minmin2,4, Meng Ju2,4, HUANG Yaojiang2,4
1. Inspection and Quarantine Technology Center of Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China;
2. Beijing Engineering Research Center of Food Environment and Public Health in Minzu University of China, Beijing 100081, China;
3. Inner Mongolia Animal Disease Prevention and Control Center of Jarud, Tongliao 029100, China;
4. Chinatex Grains & Oils (Zhanjiang) Industries Co.,LTD., Zhanjiang 524012, China
Abstract：To help identify marine fish and provide a reference standard for identification of Melanogrammus aeglefinus, a standard sample for Melanogrammus aeglefinus is established in this study. With collaboration of eight labs, concentration and quality of DNA are tested. The concentration value is (2.02±0.07) μg (k=2) and the purity value of DNA is 1.82±0.08 (k=2) which indicates good quality of samples. PCR and electrophoresis are used to test the uniformity, stability and value analysis of the samples. The results show that these samples in this study meet the technical requirements of national standards and that the method can be used for calibration and quality control of Melanogrammus aeglefinus.
蒋丹, 刘宇, 丁健, 马敏敏, 孟菊, 黄耀江. 黑线鳕鱼核酸标准样品的研制[J]. 科技导报, 2017, 35(9): 100-106.
JIANG Dan, LIU Yu, DING Jian, MA Minmin, Meng Ju, HUANG Yaojiang. The development of a Melanogrammus aeglefinus DNA standard sample. Science & Technology Review, 2017, 35(9): 100-106.
[1] 王远红, 陈四清, 吕志华, 等. 圆斑星鲽鱼的营养成分分析[J]. 营养学 报, 2006, 28(3): 271-272. Wang Yuanhong, Chen Siqing, Lv Zhihua, et al. Nutritional component analysis of Spotted halibut[J]. Journal of Nutrition, 2006, 28(3): 271-272.
[2] 于琴芳, 邓放明. 鲢鱼小黄鱼鳕鱼和海鳗肌肉中营养成分分析及评价[J]. 农产品加工刊, 2012(9): 11-14. Yu Qinfang, Deng Fangming. Muscle nutritional component analysis and evaluation of Silver carp, croaker, Gadus and conger pike[J]. Academic periodical of Farm products processing, 2012(9): 11-14.
[3] 邢薇, 罗琳, 李铁梁. 鲟鱼营养价值研究进展[J]. 中国水产, 2014(9): 70-73. Xing Wei, Luo Lin, Li Tieliang. The Nutritional value research development of Sturgeon[J]. China Fisheries, 2014(9): 70-73.
[4] 罗殷, 王锡昌, 刘源. 黄鳍金枪鱼食用品质的研究[J]. 食品科学, 2008, 29(9): 476-480. Luo Yin, Wang Xichang, Liu Yuan. The food quality research of Yellowfin tuna[J]. Food Science, 2008, 29(9): 476-480.
[5] Hawksworth D L, Kalin-Arroyo M T. Magnitude and distribution of bio-diversity[M]//Heywood V H, Watson R T. Global biodiversity assess-ment. Cambridge: Cambridge University Press, 1995.
[6] Hebert P D N, Cywinska A, Ball S L. Biological identifications through DNA barcodes[J]. Proceedings of the Royal Society of London B: Biolog-ical Sciences, 2003, 270(1512): 313-321.
[7] 林森杰, 王路, 郑连明, 等. 海洋生物DNA条形码研究现状与展望[J]. 海洋学报, 2014, 36(12): 1-17. Lin Senjie, Wang Lu, Zheng Lianming, et al. The DNA barcoding research status and prospect of Marine organisms[J]. Acta Oceanologica Sinica, 2014, 36(12): 1-17.
[8] Li D Z, Liu J Q, Chen Z D, et al. Plant DNA barcoding in China[J]. Journal of Systematics and Evolution, 2011, 49(3): 165-168.
[9] Li M, Cao H, BUT P P H, et al. Identification of herbal medicinal mate-rials using DNA barcodes[J]. Journal of Systematics and Evolution, 2011, 49(3): 271-283.
[10] 宁淑萍, 颜海飞, 郝刚, 等. 植物DNA 条形码研究进展[J]. 生物多样 性, 2008, 16(5): 417-425. Ning Shuping, Yan Haifei, Hao Gang, et al. The development research of plants DNA barcoding[J]. Biodiversity, 2008, 16(5): 417-425.
[11] Hollingsworth M L, Andra Clark A, Forrest L L, et al. Selecting bar-coding loci for plants: Evaluation of seven candidate loci with specieslevel sampling in three divergent groups of land plants[J]. Molecular Ecology Resources, 2009, 9(2): 439-457.
[12] 陈伟珠, 晋文慧, 方华, 等. 角鲨烯国家标准样品的研制[J]. 食品科 学, 2015, 36（12）: 166-170. Chen Weizhu, Jin Wenhui, Fang Hua, et al. The development squalene standard sample[J]. Food Science, 2015, 36(12): 166-170.
[13] 郑秋月, 曹际娟, 徐君怡, 等. 转基因玉米质粒分子国家标准样品的 构建[J]. 标准科学, 2012(12): 48-51. Zheng Qiuyue, Cao Jijuan, Xu Junyi, et al. The construction of genetically modified corn plasmid national standard samples[J]. Standard Science, 2012(12): 48-51.
[14] Hubalkova Z, Kralik P, Kasalova J, et al. Identification of gadoid spe-cies in fish meat by polymerase chain reaction (PCR) on genomic DNA[J]. Journal of agricultural and food chemistry, 2008, 56(10): 3454-3459.
[15] Guide ISO. General requirements for the competence of reference me-terial producers[S]. Geneva: International Orgnization for standardiza-tion, 2000.
2017, Vol. 35 
