The development of a Melanogrammus aeglefinus DNA standard sample
JIANG Dan1, LIU Yu2, DING Jian1, MA Minmin2,4, Meng Ju2,4, HUANG Yaojiang2,4
1. Inspection and Quarantine Technology Center of Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China;
2. Beijing Engineering Research Center of Food Environment and Public Health in Minzu University of China, Beijing 100081, China;
3. Inner Mongolia Animal Disease Prevention and Control Center of Jarud, Tongliao 029100, China;
4. Chinatex Grains & Oils (Zhanjiang) Industries Co.,LTD., Zhanjiang 524012, China
Abstract:To help identify marine fish and provide a reference standard for identification of Melanogrammus aeglefinus, a standard sample for Melanogrammus aeglefinus is established in this study. With collaboration of eight labs, concentration and quality of DNA are tested. The concentration value is (2.02±0.07) μg (k=2) and the purity value of DNA is 1.82±0.08 (k=2) which indicates good quality of samples. PCR and electrophoresis are used to test the uniformity, stability and value analysis of the samples. The results show that these samples in this study meet the technical requirements of national standards and that the method can be used for calibration and quality control of Melanogrammus aeglefinus.
蒋丹, 刘宇, 丁健, 马敏敏, 孟菊, 黄耀江. 黑线鳕鱼核酸标准样品的研制[J]. 科技导报, 2017, 35(9): 100-106.
JIANG Dan, LIU Yu, DING Jian, MA Minmin, Meng Ju, HUANG Yaojiang. The development of a Melanogrammus aeglefinus DNA standard sample. Science & Technology Review, 2017, 35(9): 100-106.
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