明日叶查尔酮对紫外线诱发HaCaT细胞损伤的防护作用

doi:10.3981/j.issn.1000-7857.2019.14.012

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摘要为研究明日叶查尔酮（Angelica keiskei chalcones，AC）对紫外线诱发HaCaT细胞损伤的防护作用，采用紫外线照射损伤的HaCaT细胞，分别加入高、中、低剂量依次为20、10、5μmol/L终浓度的AC，共同培养24 h，采用3-（4，5-dimethyl-2-thiazolyl）-2，5-diphenyl-2-Htetrazolium bromide（MTT）比色法测定细胞增殖活性，流式细胞仪测定细胞凋亡率，试剂盒法测定细胞MDA含量和SOD、CAT及Caspase-3的活性，逆转录聚合酶链式反应方法检测细胞BCL-2和Bax的mRNA表达水平。结果表明，与正常对照组比较，照射模型组细胞增殖活性、SOD、CAT活性、BCL-2 mRNA表达水平降低，而细胞MDA含量、凋亡率、Caspase-3活性以及Bax mRNA表达水平则升高；中、高剂量AC组细胞增殖活性、SOD、CAT活性、BCL-2mRNA表达水平均高于照射模型组，而细胞MDA含量、凋亡率、Caspase-3活性以及BaxmRNA表达水平则均低于照射模型组。上述各项差别均具有统计学意义（P<0.05）。研究发现，明日叶查尔酮可抑制紫外线照射诱发的HaCaT细胞凋亡，调节BCL-2和Bax的mRNA表达水平，增强细胞的抗氧化酶活性，减轻受损细胞的脂质过氧化水平，对紫外线照射导致的细胞损伤有良好防护作用。

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	李蕾
	孔杭如
	钟文雯

关键词 ：明日叶查尔酮, HaCaT细胞, 氧化损伤, 凋亡

Abstract：The protective effect of Angelica keiskei chalcones on the damage of HaCaT cells induced by ultraviolet B(UVB) is investigated.. HaCaT cell with oxidative damage induced by ultraviolet B is incubated with Angelica keiskei chalcones at concentrations of 20 μmol/L, 10 μmol/L and 5 μmol/L for 24 h. The cell viability as well as the apoptosis rate are determined by MTT method and flow cytometer, respectively. The MDA content of cells and activities of SOD, CAT and Caspase-3 are determined by kit method. The mRNA expression of BCL-2 and Bax is evaluated by reverse transcription polymerase chain reaction (RT-PCR). Compared with the normal control, the cell viability, SOD, CAT activity and the expression level of BCL-2 mRNA in the irradiation model group are decreased while the MDA content, apoptosis rate, Caspase-3 activity and Bax mRNA are increased. The viability, SOD, CAT activity and BCL-2 mRNA expression of cells incubated with 20 μmol/L and 10 μmol/L are increased while the apoptosis rate, Caspase-3 activity and Bax mRNA expression are decreased, compared with the irradiation model group. All the differences are significant (P<0.05). Angelica keiskei chalcones may protect HaCaT cells from oxidative damage and decrease apoptosis rate by regulating the mRNA expression of BCL-2 and Bax.

Key words： Angelica keiskei chalcones HaCaT cell oxidative damage apoptosis

收稿日期: 2018-07-06

基金资助:福建省自然科学基金项目（2014J05098）

作者简介: 李蕾,副教授,研究方向为营养资源的开发与应用,电子信箱:Lilei2003@126.com;孔杭如(共同第一作者),硕士研究生,研究方向为天然活性物质的开发与应用,电子信箱:278976774@qq.com

引用本文:

李蕾, 孔杭如, 钟文雯. 明日叶查尔酮对紫外线诱发HaCaT细胞损伤的防护作用[J]. 科技导报, 2019, 37(14): 93-99.
LI Lei, KONG Hangru, ZHONG Wenwen. The protective effect of Angelica keiskei chalcones on the damage of HaCaT cells induced by ultraviolet B. Science & Technology Review, 2019, 37(14): 93-99.

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http://www.kjdb.org/CN/10.3981/j.issn.1000-7857.2019.14.012 或 http://www.kjdb.org/CN/Y2019/V37/I14/93

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