Abstract：Human Lactoperoxidase gene (hLPO) has been cloned into pBC1 to construct pBC1-hLPO specialized expression vector in mammary glands. Human LPO gene was assembled by two gene fragments, which are 3 kilo base pairs in length and 27 kilo base pairs long respectively. The shorter one was amplified by long-distance PCR and the other was cut down by restriction AgeI, both based on the donor of PAC RP5-1171I10 plasmid that contains hLPO gene. Here, We established the transgenic mouse model by microinjection into fertilized eggs, and then obtained 5 transgenic mice (4male, 1female) out of 28 descendants, identified by PCR and Southern blot. The integration rate is 17.86%, and lactoperoxidase expression levels were determined by SDS-PAGE gel electrophoresis and Western blot. The hLPO recombinant protein band is not obvious as the antibodies are not very specific.
2005, Vol. 23 
