Abstract：Restriction endonuclease PCR method was utilized to analyze 33 cases tumor tissue and 15 cases normal tissue. The data was analyzed by SPSS Software. At last, it shows that there are 18 cases cervical tumor methyltion in SacⅡ cleavage site and 8 cases cervical tumor methyltion in SmaⅠ cleavage site, on the contrary, there are 2 normal tissue methyltion in SacⅡ cleavage site and no normal tissue methyltion in SmaⅠ cleavage site. In addition, the early stage cervical tumor are more ease methyltion in those two CpG inland. In a word, the exon 1 of p16 gene SacⅡand SmaⅠCpG island methylation related with cervical tumor carcinogenesis.