Abstract：To investigate effects of synthetic oligodeoxynucleotide containing CpG motif (CpG ODN) on specific antibody production and lymphocyte proliferation to recombinant hepatitis B surface antigen (rHBsAg). Methods: Balb/c mice received into back leg tibialis anterior (TA) muscle for two times;Detection of anti-HBs titer in serum by ELISA;The lymphocyte proliferations are tested by 3H-TdR incorporation. Results: The anti-HBs titer of plus CpG ODN group is higher than that of rHBsAg group obviously, and the antibody persistent time is also longer. Nevertheless, the proliferation of splenocytes of the group plus CpG ODN is much stronger. Conclusion: CpG ODN has enhanced efficacy for anti-HBs production and stimulated the splenocytes proliferation in mice.
2005, Vol. 23 
