Abstract:The treatment of coal wastes in coal mine areas is a difficult issue. A strain of chemolitho autotrophic sulfur oxidizing bacteria was isolated from coal wastes in the lab to study its desulphurization effects on coal wastes coming from Dawukou coal mine areas. The pole leaching experiment was conducted in natural conditions. The results show that the microbe can promote sulfur oxidation in the coal wastes. It also can reduce the pH value and increase electricity conductivity (EC) in the leaching solution. The iron was emerged from the coal wastes. The microbe can shorten the desulphurization periods. The sulphate concentration was enhanced after leaching 4 times. The desulphurization rate can reach 67.6% and its desulphurization distribution rate is 11% after leaching 15 times. EC and pH values were measured in the coal wastes after desulphurization. pH value was increased and EC decreased significantly in the coal wastes medium. That will make the coal wastes more stable in their properties, which may contribute to the ecological restoration with respect to the coal wastes and promote environmental sustainable development in the coal mine areas.