利用中国矿业大学（北京）微生物复垦实验室自主分离筛选出的嗜酸氧化硫硫杆菌，模拟自然堆放条件，采用柱状淋滤脱硫试验来研究其对大武口高硫煤矸石的脱硫效果。结果表明，脱硫微生物能够促进煤矸石中硫的氧化，使淋滤液pH值降低，电导率明显增加，煤矸石中的盐基离子被脱出来，脱硫微生物缩短了煤矸石的脱硫周期，在淋滤前4次脱出的硫酸根浓度增加明显。经过15次淋滤后，煤矸石脱硫量达到67.64%，氧化硫硫杆菌对脱硫的贡献率为11.08%。经过脱硫后煤矸石基质的pH值有所提高，盐分含量明显下降，矸石的性状趋于稳定，可为煤矿区矸石山的生态恢复奠定基础，促进煤矿区环境的可持续发展。

The treatment of coal wastes in coal mine areas is a difficult issue. A strain of chemolitho autotrophic sulfur oxidizing bacteria was isolated from coal wastes in the lab to study its desulphurization effects on coal wastes coming from Dawukou coal mine areas. The pole leaching experiment was conducted in natural conditions. The results show that the microbe can promote sulfur oxidation in the coal wastes. It also can reduce the pH value and increase electricity conductivity (EC) in the leaching solution. The iron was emerged from the coal wastes. The microbe can shorten the desulphurization periods. The sulphate concentration was enhanced after leaching 4 times. The desulphurization rate can reach 67.6% and its desulphurization distribution rate is 11% after leaching 15 times. EC and pH values were measured in the coal wastes after desulphurization. pH value was increased and EC decreased significantly in the coal wastes medium. That will make the coal wastes more stable in their properties, which may contribute to the ecological restoration with respect to the coal wastes and promote environmental sustainable development in the coal mine areas.