Abstract：To study the effects of 5'-nucleotides on modulation of adaptive immunity of mice and to determine the best dosage, female BALB/c mice were administered with common control diet, Nucleotides-Free(NF) diet and NF diet with 0.0025%, 0.01%, 0.04%, 0.16% 5'-nucleotides for 4 weeks. Cellular immune functions, humoral immune functions, the level of immunoglobulin in serum and splenic T cell subpopulations of the spleen tissue by the flow cytometer were examined. The results show that with the NF diet, the capacity of lymphocyte proliferation induced by ConA (0.06±0.03) and DTH response (0.12±0.06)mm are significantly reduced; the ratio of CD4+/CD8+ is decreased (1.90±0.12) while Tr percentage is increased (5.56±0.11). A decrease trend of B lymphocytes antibody response was observed. The NF diet with 5'-nucleotides can prevent these decreases. At the dose of 0.04%, the immunological functions are increased significantly as compared with those of NF group, reaching normal values. The results indicate that the NF diet can impair the immunological function of mice, while the NF diet with 5'-nucleotides can prevent the decrease, and 0.04% is the best dose.
2010, Vol. 28 
