Abstract：To investigate the isolation method of Dorsal Root Ganglion(DRG) neurons in rats and their electrophysiological characteristics, DRGs were obtained by double enzymatic dissociation. The patch clamp technique was used to record Action Potentials (AP) and sodium currents INa. The results show that a body of round and long DRG, and DRG neurons with middle measures and clear membranes and nucleus was obtained with good refraction properties. APs recorded in DRG neurons consist of five phases and in shape of upward acute triangles, with small static potential and short action duration. INa for DRG neurons, as the peak current density, is equal to (-62.04±4.45) pA/pF, and it is almost inhibited by tetrodotoxin and recovered from the inactivated state. It is concluded that the operations on the body of DRG are simple and easy to handle; DRG neurons are obtained quickly and identified; and the electrophysiological characteristics of DRG neurons are typical and clear. The methods and results may be used in investigations on electrophysiological properties and in medical treatments for diseases in nervous system.
2009, Vol. 27 
