Abstract：The spatial positional memory span represents the human perception of spatial orientation and short-time memory, which is significant for people with short-memory spans. However, studies are needed to investigate these problems, such as (1) whether there is a relationship between memory span and memorization of numbers or wrong numbers; (2) whether different experiment conditions affect spatial memory; and (3) whether there are differences in spatial positional memory between sexes. Therefore, in this paper, 100 subjects participated in two experimental paradigms to answer the above questions. We found that, in the same coding condition, there is a linear regressive relationship between human spatial positional memory span with memorization of numbers and wrong numbers. In the different coding condition, there were distinct differences in spatial positional memory between each condition; however, there were no differences between sexes.