Abstract:Ovarian granulosa cells from porcine follicles were isolated and cultured in vitro for determination of the insulin resistance, by expressions of insulin signal molecules and gonadotropin-releasing hormone signal transduction, and to establish the relations between glucose metabolic disorders and reproductive dysfunction in polycystic ovary syndrome. A comparison was made between insulin-sensitizing western medicines and two traditional Chinese medicines berberine (Ber) and daidzein (Dai) in their improvement effects on insulin sensitivity, and the effects on function of the ovarian granulose cells.