Abstract：If the rat model of diabetes-Akt2 gene knockout mice is homozygous (Akt2 (-/-)) for ovarian function and fertility, then it would be proved that Akt2 gene could affect ovary function and glucose and lipid metabolism. In the experiment, heterozygote (Akt2 (+/-)) of male and female mice is cross-breeding for 60 days, then weanling offspring have been given gene identification in order to select the wild-type (Akt2 (+/+)) and homozygous (Akt2 (-/-)) mice for breeding. As the son of second generation female, subjects are that observe body and fat weight, the estrous cycle, glucose, hormone changes, ovary, and ovary morphological changes. The results show that: (1) homozygous weight and body fat weight of the mice are compared with that of wild type, but no difference in statistics; (2) Akt2 (-/-) decline in fertility (P<0.05), the estrous cycle (P<0.05) is extended, the existence of abnormal ovarian function is promoted. (3) 0h and 2h, random blood glucose, fasting insulin and HOMA index, and homozygote are higher than that of wild-type (P<0.05); (4) Akt2 (-/-) serum 17-hydroxy progesterone (17-OHP), estradiol (E2), 17-OHP, E2 are increased; (5) for the lipid levels,, basic group of high-density lipoprotein (HDL), triglyeride (TG) (P<0.05) in homozygote are lower than that of wild-type. In conclusion, Akt2 gene can affect glucose metabolism and ovarian function, insulin which is a key signaling molecule regulation of glucose metabolism plays an important role in ovarian function.
2011, Vol. 29 
