Abstract：Survivin siRNA is transfected to colon cancer cells SW480 and the interference on proliferation and apoptosis is studied to provide the experimental basis for gene therapy of colon cancer and seek new, effective RNA interference fragment. Three siRNA targeting Survivin gene were constructed and transfected into SW480. After 24, 48, and 72h, the change of survivin protein was detected by using Western blot, in the same time, cell proliferation was detected by using MTT assay. The results show that after three siRNA effect on SW480 cells at 24, 48, and 72h, three groups of Survivin protein expression are all appeared the decrement (P<0.05). The expression of Survivin protein reached apex at 48h and the inhibitory rates were 66.5%±2.1%, 49.6%±2.8% and 47.8%±3.1%, respectively, compared with the control group (P<0.05). MTT assay show that the cell proliferation was inhibited after Survivin gene silencing. Liposome-mediated Survivin siRNA can effectively silence the Survivin gene in colon cancer cells, therefore inhibit cancer cell proliferation and promote apoptosis, it is especially designed for targeting conservative regions of siRNA, and interference effects are more significant.