Abstract：To investigate the millimeter wave chondrocyte intervention activity of β-catenin and its mechanism. Methods: SD rats with knee joint cartilage, the establishment of in vitro cultured chondrocyte, identification of toluidine blue staining. No. 2 on behalf of chondrocyte culture 48h, serum-free DMEM with hunger 24h, randomized, using different times after the intervention of the millimeter wave detection of the indicators. Results: The intervention in each group before and after the chondrocyte of type Ⅱ collagen expression and stability can be sustained; experiment 2, experiment 3 groups of chondrocyte β-catenin, CKIε expression was significantly higher than the normal group, experimental group 1 (P <0.05, P < 0.01), experiment 2, experiment 3 groups of chondrocyte l, GSK-3β expression was significantly lower than the normal group, experimental group 1 (P <0.05, P <0.01). Conclusion: The millimeter wave may be induced by transcriptional synthesis of chondrocyte, β-catenin, CKIε, inhibit the expression of GSK-3β, thereby promoting synthesis of β-catenin, inhibit decomposition of chondrocyte to enhance β-catenin activity.