Effects of Fastigial Nucleus Electrical Stimulation on Calcium-overload in Myocardium of Rats after Myocardial Infarction
ZHANG Runfeng1, LI Xia2, HU Dayi3, GAO Wengen3, WEI Yidong4, XING Yan4
1. Department of Cardiology, Third People's Hospital of Mianyang, Mianyang 621000, Sichuan Province, China;2. Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, China;3. Heart Center, Peking University People's Hospital, Beijing 100044, China;4. Heart, Lung and Blood Vessel Center, Tongji University, Shanghai 200092, China
Abstract：Exploring the effect of Fastigial Nucleus Stimulation (FNS) on intracellular calcium overload in rats after Myocardial Infarction (MI) is taken aim at. Ninety SD rats were randomly divided into three groups with 30 rats for each group, that is, MI group, FNS group, and fastigial nucleus lesion group. In the MI group, left anterior descending artery of rats was ligated. In the FNS group, rats were pretreated with fastigial nucleus electro-stimulation for 1h, and then left anterior descending artery was ligated. In the fastigial nucleus lesion group, rats were treated with fastigial nucleus electro-stimulation for 1h after their fastigial nucleus was damaged for 5d, and then left anterior descending artery was ligated. Each group was further divided into three subgroups according to the three time points of 1, 7, and 21d, respectively after the ligation of left anterior descending artery. Eight SD rats were chosen as the sham operation group． After 1, 7, and 21d with the ligation of left anterior descending artery, the left ventricular myocytes were isolated from rat hearts. The Ca2+ concentration was determined by using laser scanning confocal microscopy. The results indicate that the fluorescence intensity of the labeled intracellular Ca2+ in MI group and fastigial nucleus lesion group is significantly higher than that in sham-operated group at the day one and day seven after MI (P<0.05), however the intensity in FNS group was significantly lower than that in MI group (P<0.05). There is no significant difference in Ca2+ concentration between MI group and fastigial nucleus lesion group (P>0.05). At the day 21 after MI, there are no significant differences in Ca2+ concentration among these four groups. Therefore, it might be concluded that FNS could decrease the Ca2+ concentration in myocytes at an early stage after MI, and might control MI mortality by inhibiting intracellular Ca2+ overload.
2011, Vol. 29 
