Effects of Xipayi Mouth Rinse on the PGE2 Production of Human Gingival Fibroblast (HGF) Induced by IL-1β
YUSUF Abibai1, AIZEZI Renaguli2, UPUR Halmurat2
1. Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China;2. Department of Uygur Medicine, Xinjiang Medical University, Urumqi 830054, China
Abstract:To observe the effects of Xipayi mouth rinse on the prostaglandin E2 (PGE2) production of Human Gingival Fibroblast (HGF) by stimulation of interleukin-1β (IL-1β), the HGF cells cultured by healthy human gingival tissue are used. With 1ng/mL IL-1β as a stimulating factor, HGF cells with 5×104/mL cell density were inoculated into a culture plate with 24 holes, each containing 200μL cell suspension. 24h after incubation and adhering to the wall, the original culture liquid was abandoned and the mouth was washed 2 times after adding the drugs, which were grouped into control group (with 20m/L fetal bovine serum medium DMEM), IL-1β group (beta concentration is 1ng/mL), IL-1β+Xipayi mouth rinse group (end concentrations of were 50, 100, 200g/mL, respectively ), IL-1β+indomethacin (100ng/mL of endcentration) group. The enzyme immunoassay method is used to check the PGE2 and observe the effects of different concentrations of Xipayi mouth rinse on PGE2 levels in the HGF supernatant. Results show that Xipayi mouth rinse at different concentrations suppresses the PGE2 production of HGF stimulated with 1ng/mL IL-1β significantly in a dose dependent manner. It is concluded that the human gingival fibroblast has the ability of synthesizing and secreting PGE2, and IL-1β is a potent stimulation for HGF cell to generate PGE2; and Xipayi mouth rinse can significantly inhibit the PGE2 production of HGF by stimulation of IL-1β.
艾比拜·玉素甫;热娜古丽·艾则孜;哈木拉提·吾甫尔. 西帕依固龈液对IL-1β刺激人牙龈成纤维细胞产生PGE2的影响[J]. , 2012, 30(15): 57-60.
YUSUF Abibai;AIZEZI Renaguli;UPUR Halmurat. Effects of Xipayi Mouth Rinse on the PGE2 Production of Human Gingival Fibroblast (HGF) Induced by IL-1β. , 2012, 30(15): 57-60.