本文通过对76例哮喘患者按维医体液论进行辨证分型,其中异常黑胆质性哮喘组30例、非异常黑胆质性哮喘组46例、正常对照组89例,采用聚合酶联反应——限制性片段长度多态性(PCR-RFLP)技术检测各组IL-13基因intron3+1923位点及IL-13基因+2044位点的多态性,来探讨新疆维吾尔族异常黑胆质性哮喘与白细胞介素-13(IL-13)基因多态性间的关系.结果发现IL-13基因intron3+1923位点多态性,在三组间有显著性差异(P＜0.01),基因型频率异常黑胆质性哮喘组TT、TC基因型分布频率明显高于非异常黑胆质性哮喘组和正常对照组(P＜0.01),非异常黑胆质性哮喘组TT、TC基因型分布频率高于正常对照组(P＜0.05),而正常对照组CC基因型分布频率明显高于异常黑胆质性哮喘组(P＜0.01)和非异常黑胆质性哮喘组(P＜0.05),非异常黑胆质性哮喘组CC基因型分布频率高于异常黑胆质性哮喘组(P＜0.05),等位基因T、C的频率分布在三组人群中有差异(P＜0.01).T等位基因频率分布异常黑胆质性哮喘组高于正常对照组(P＜0.01).C等位基因分布频率异常黑胆质性哮喘组低于正常对照组(P＜0.05).IL-13基因+2044位点基因型频率及等位基因频率分布在各组间比较无差异(P＞0.05).结果表明,新疆维吾尔族异常黑胆质性哮喘可能与IL-13基因intron3+1923位点多态性有关,而与IL-13基因+2044位点无关.

According to the body fluid theory of Uygur Medicine, 76 cases of asthmatic patients were diagnosed. Among them, there were 30 cases of asthmatic patients with abnormal savda and 46 cases of asthmatic patients with normal savda. The gene polymorphism of IL-13 on loci intron3+1923 and loci +2044 of these patients are tested by using PCR-RFLP, the results are compared with that of the 89 normal individuals in order to explore the relationship between asthmatic Xinjiang Uygur patients with abnormal savda and the gene polymorphism of IL-13. There is significant difference of gene polymorphism of IL-13 on loci intron3+1923 in all three groups(P＜0.01). The distribution rate of genotype TT and TC in asthmatic patients with abnormal savda is significantly higher than that of the asthmatic patients with normal savda and the normal control group(P＜0.01). The distribution rate of genotype TT and TC in asthmatic patients with normal savda is significantly higher than that of the normal control group (P＜0.05). The distribution rate of genotype CC in normal control group is significantly higher than that of the asthmatic patients with abnormal savda(P＜0.01) and higher than that of the asthmatic patients with abnormal savda (P＜0.05). The distribution rate of genotype CC in asthmatic patients with normal savda is significantly higher than that of the asthmatic patients with abnormal savda(P＜0.05). There is significant difference in the distribution rate of allele C and T in all three groups(P＜0.01). The distribution rate of allele T in asthmatic patients with abnormal savda is significantly higher than that of the normal control group(P＜0.01). The distribution rate of allele C in asthmatic patients with abnormal savda is significantly lower than that of the normal control group(P＜0.05). There is insignificant difference in the distribution rate of IL-13 on loci +2044 and the distribution rate of its alleles in all three groups(P＞0.05). In short, Asthma in Xinjiang Uygur Patients with abnormal savda might be related to the gene polymorphism of IL-13 loci intron3+1923, but is not related to the gene polymorphism of IL-13 on loci+ 2044.