为了观察异黑颗粒对小鼠行为学习及抗氧化能力的影响,选用APP/PS1双转基因模型小鼠,将其随机分成5组,另取同窝阴性小鼠作为正常对照组,每组8只,3个月龄后开始给药.给药6个月后,通过行为学实验测试小鼠学习记忆能力的变化,采用分光光度法测定脑组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性.Morris水迷宫实验结果表明,APP/PS1双转基因模型小鼠逃避潜伏期较正常组明显延长,在原象限停留时间明显缩短;异黑颗粒组小鼠逃避潜伏期较模型组明显缩短,在原象限停留时间明显延长.APP/PS1双转基因模型小鼠脑组织GSH-Px、SOD活力下降;异黑颗粒治疗组学习和记忆能力升高,脑组织GSH-Px、SOD活力升高,与阿尔茨海默病模型组比较有显著性差异.研究表明,APP/PS1双转基因模型小鼠9个月后发现有明显学习记忆功能障碍伴有抗氧化能力下降,异黑颗粒对阿尔茨海默病模型小鼠学习记忆障碍具有明显改善作用并且可剂量依赖性的提高阿尔茨海默病模型小鼠的抗氧化能力.

In order to study the effect of savda granule on memory and antioxidation ability for APP/PS1 double transgenic mice, a number of three-month-old APP/PS1 double transgenic mice were randomly divided into five groups. Meanwhile, the mice with negative control were served as a control group. There were eight mice in each group and all of them were intragastrically administered for six months. Then the learning and memory ability of mice were detected by Morris water maze test. The activities of glutathione peroxidase-(GSH-Px) and superoxide in the brain tissue were measured with spectrophotometer. Comparing with the control group, APP/PS1 double transgenic mice significantly prolong the latency for searching the hidden platform in the directional swimming test, and decrease the time spent on swimming in the target quadrant with the probe test. Comparing with APP/PS1 double transgenic mice, savda granule is able to dose-dependently shorten the latency for searching the hidden platform in the directional swimming test, and increase the time spent on swimming in the target quadrant with the probe test. The activities of GSH-Px and SOD decrease in APP/PS1 double transgenic mice, while in the groups with savda granule treatment, the activities of GSH-Px and SOD raise, and there is significant difference comparing with Alzheimer's Disease (AD) model group. Nine-month-old APP/PS1 double transgenic mice present imitated learning and memory dysfunction with the reduction of antioxidation ability; savda granule is able to ameliorate the impaired memory function for the mice with AD and improve their antioxidation ability.