研究论文

基于磁分离的高通量核酸检测工作站控制系统设计

  • 万遂人;程铅;何农跃;刘宾
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  • 1. 东南大学生物科学与医学工程学院,南京 210096;2. 南京医科大学基础医学院生物医学工程系,南京 210029

收稿日期: 2013-01-11

  修回日期: 2013-02-26

  网络出版日期: 2013-05-08

Control System of High-throughput Nucleic Acid Detection Workstations Based on Magnetic Separation

  • WAN Suiren;CHENG Qian;HE Nongyue;LIU Bin
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  • 1. School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China;2. School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China

Received date: 2013-01-11

  Revised date: 2013-02-26

  Online published: 2013-05-08

摘要

核酸检测工作站主要是由光、机、电等部分构成的高精度生化分析仪器,其功能模块主要有:高通量磁分离系统、微量移液系统、精确温控系统、荧光信号检测系统.根据以上系统应用要求,简要介绍了系统硬件框架.基于硬件基础,系统软件采用树形控件完成了实验流程设计;采用MFC绘图完成了美观、易操作的实验属性设置和工作台布局设置,采用多线程监控技术实现与下位机RSC-485串口通信;采用Access数据库保存实验流程及结果,并导出打印实验数据等.

本文引用格式

万遂人;程铅;何农跃;刘宾 . 基于磁分离的高通量核酸检测工作站控制系统设计[J]. 科技导报, 2013 , 31(13) : 54 -57 . DOI: 10.3981/j.issn.1000-7857.2013.13.008

Abstract

The nucleic acid detection workstation is a high-precision biochemical analytical instrument composed of optical, mechanical, electrical and other components. There are four functional modules in the workstation, including the high throughput magnetic separation system, the micro-volume pipetting system, the accurate temperature control system and the fluorescent signal detection system. According to the requirements of the above systems, a brief introduction is given for the framework of the hardware system. Based on the hardware functions, the software system completes the design of the experimental process using the tree control: an easy to use and good looking experimental property setting interface and a workbench layout with the MFC drawing. Serial communications are carried out through the slave PC's RSC-485 port with multi-threaded monitoring technologies: saving the experimental processes and results, exporting experimental data with the access database programming for their printing.
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