为建立裸鼠胫骨原位骨肉瘤模型,并比较分析MG-63细胞悬液局部注射以及肿瘤组织块移植两种方法的成瘤特性,在注射组体内连续传代筛选MG-63细胞系获得高成瘤率的细胞,将MG-63细胞悬液(25μL,约1×105个)注射于裸鼠胫骨上段髓腔内;组织块移植组将MG-63细胞皮下成瘤瘤组织块接种于裸鼠胫骨上段髓腔内。随后观察两种方法建立的胫骨骨肉瘤原位模型不同的成瘤特性,并采用影像学等手段比较两种方法的成瘤率和成瘤特点。结果表明,术后3周左右可见局部肿瘤形成,4周后通过小动物X线技术可见胫骨中上段溶骨与肿瘤样骨形成,同时在光学显微镜下可见典型的骨肉瘤病理特征。细胞悬液法成瘤率85%,组织块法成瘤率95%,两种方法成瘤率的差异有统计学意义(P<0.05),模型组血清碱性磷酸酶(ALP)明显高于对照组(P<0.01)。两种方法均可形成裸鼠胫骨原位骨肉瘤模型,采用组织块移植法成瘤率较高,瘤体生长速度较快,而且对骨皮质及周围软组织的破坏力较强,转移率高。
To establish an osteosarcoma model of the nude mouse osteosarcoma, and to compare the cell suspension shots and the tissue mass transfer of the two methods with respect to the tumor characteristics, the culture is continuously transferred into an injection group body and the MG-63 is screened to obtain the Du145 cell line with a top rate of tumor cells. The MG-63 cell suspension (25μL, about 1×105) is injected into the nude mouse tibial cavity; The organization block transplantation group will transplant the MG-63 cells into the subcutaneous tumor with the tumor tissue mass vaccination in the nude mouse tibial top. Then the two methods will be compared in the tibia bone sarcoma in the situ model with respect to the tumor characteristics, and the imaging methods are used to compare the tumor rate and the tumor characteristics. Postoperative has revealed after 2-3 weeks the visible local tumor formation, after 4 weeks in the X ray film the visible tibia of soluble tumor samples of bone formation above the average period, and at the same time in the lens the typical osteosarcoma pathological features. With the cell suspension method, the tumor rate is 85%, with the organization block method, the tumor rate is 95%, and their difference is significant (P<0.05). The serum alkaline phosphatase of the model group is obviously higher than that of the blank control group (P<0.01). With both methods, the nude mouse tibia can be formed in the in-situ osteosarcoma model, but with the method of organization block transplant, the tumor rate is higher, the tumor growth is faster, and the surrounding soft tissue damage is stronger, closer to human tumors with respect to the transfer characteristics as well.
