通过检测急性毒性实验中含甘遂甘草反药组合的甘遂半夏汤及其拆方各组中未死亡小鼠的肝功能和肾功能的情况,以期为急性毒性实验的结果提供相关的依据。将18~20g的昆明小鼠随机分为空白组、甘遂半夏汤组、甘遂半夏汤去炙甘草组、甘遂半夏汤去醋甘遂组、甘遂半夏汤去炙甘草醋甘遂组、炙甘草组、醋甘遂组、炙甘草配醋甘遂组,每个给药组以1:0.85的比例稀释为5个剂量组,空白组灌胃蒸馏水,其余各组灌胃给药,连续给药28d,摘除未死亡小鼠的眼球取血,离心,检测血清中的丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、肌酐(CRE)、尿素氮(BUN)水平。AST结果显示,与空白组相比,甘遂半夏汤组1、2和3,甘遂半夏汤去醋甘遂组2、3和5,甘遂半夏汤去炙甘草醋甘遂组4,炙甘草组2、3、4和5,醋甘遂组1、3、4和5,炙甘草配醋甘遂组2、3和5血清中AST的活性均高于空白组且具有统计学差异。ALT结果显示,与空白组比较,醋甘遂组4血中ALT的活性显著高于空白组(P<0.01),甘遂半夏汤去炙甘草组4低于空白组,且具有统计学差异(P<0.05),炙甘草组2,醋甘遂组1、3、5,炙甘草配醋甘遂组1、2、3和5这8组血中ALT的活性显著高于空白组且具有统计学差异(P<0.05),其余各组血中ALT的活性与空白组比较不具有统计学差异(P >0.05)。ALP结果显示,与空白组比较,各组血中ALP的活性与空白组比较不具有统计学差异。BUN结果显示,与空白组比较,甘遂半夏汤组5,甘遂半夏汤去炙甘草组2、5,甘遂半夏汤去醋甘遂组2、3,甘遂半夏汤去炙甘草醋甘遂组1、2,这7组血中BUN的含量较空白组显著降低(P<0.01),甘遂半夏汤去醋甘遂组4,甘遂半夏汤去炙甘草醋甘遂组3、5,醋甘遂组2血中BUN的含量低于空白组且具有统计学差异(P<0.05),LD_50(n)实验中未死亡小鼠血中BUN含量降低是否属于肾损伤还有待于实验进一步验证。CRE结果显示与空白组比较,各组血中CRE的含量与空白组比较不具有统计学差异(P >0.05)。由此可以看出,LD_50(n)实验中,各组药物对小鼠肝肾功能的影响主要表现为对肝脏具有一定程度的损伤,且甘遂甘草反药组合在复方中应用较二者单独配伍应用对肝脏的损伤减小;LD_50(n)实验中,含甘遂甘草反药组合的复方在一定剂量及给药时间下表现出对未死亡小鼠肝功能的损伤有可能来自于甘遂甘草反药组合。

By the detection of the liver function and renal function of mice in acute toxicity test that were given Gansuibanxia decoction that adds or subtracts Gansui and Gancao anti-drug combination, we expect that these results provide evidence for acute toxicity experiments. The male Kunming mice of 18-20 g were randomly grouped, including the blank group, the group of Gansuibanxia decoction, the group of Gansuibanxia decoction with Zhigancao removed, the group of Gansuibanxia decoction with Cugansui removed, the group of Gansuibanxia decoction with Zhigancao and Cugansui removed, the group of Zhigancao, the group of Cugansui,the group of compatibility of Zhigancao and Cugansui. Each administration group was divided into five dose groups by diluting to a ratio of 1:0.85. Blank group was given distilled water, the rest groups were given the drugs. The method of administration was maintained for 28 days, then the eyes of the mice that did not die were removed and centrifugated, to detect serum alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP), creatinine(CRE), blood urea nitrogen(BUN) levels. The AST results show that, compared with the blank group, the serum AST activity of the group of Gansuibanxia decoction 1, 2 and 3, the group of Gansuibanxia decoction with Cugansui removed 2, 3 and 5, the group of Gansuibanxia decoction with Zhigancao removed and Cugansui 4, the group of Zhigancao 2, 3, 4 and 5, the group of Cugansui 1, 3, 4 and 5, the group of compatibility of Zhigancao and Cugansui 2, 3 and 5 were higher than the blank group and had statistically difference. The ALT results show that compared with the blank group, the serum ALT activity of the group of Cugansui 4 was higher than the blank group(P<0.01). The serum ALT activity of the group of Gansuibanxia decoction with Zhigancao removed 4 was lower than blank group(P<0.05). The serum ALT activity of the group of Zhigancao 2, the group of Cugansui 1, 3 and 5, the group of compatibility of Zhigancao and Cugansui 1, 2, 3 and 5 were higher than the blank group(P<0.05). The blood ALT activity of the rest groups in comparison with the blank group does not have a statistically significant difference(P >0.05). The ALP results show that the serum ALP activity of all the groups does not have a statistically significant difference compared with the blank group. The BUN results show that compared with the blank group, the serum BUN activity of the group of Gansuibanxia decoction 5, the group of Gansuibanxia decoction with Zhigancao removed 2 and 5, the group of Gansuibanxia decoction with Cugansui removed 2 and 3, the group of Gansuibanxia decoction with Zhigancao and Cugansui removed 1 and 2 were lower than the blank group(P<0.01). The serum BUN activity of the group of Gansuibanxia decoction with Cugansui removed 4, the group of Gansuibanxia decoction with Zhigancao and Cugansui removed 3 and 5, the group of Cugansui 2 were lower than the blank group(P<0.05). BUN decreased in the blood of mice that did not die in the experiment of LD_50(n), and it is a kidney injuryhas yet to be further experimentally verified. The CRE results show that all of the blood CRE content in comparison with the blank group did not have a statistically significant difference(P >0.05). It can be seen that in the experiment of LD_50(n), each group of drugs on the liver and kidney of mice mainly has a certain degree of damage to the liver, and the liver injury of anti-drug combination of the Gansui and Gancao application in the compound compared with two separate application is decreased. In the LD_50(n) experiments, the compound containing anti-drug combination of Gansui and Gancao in a certain dose and time of administration showed a certain liver damage of not died mice may come from the anti-drug combination of Gansui and Gancao.