为了研究低强度脉冲超声促进MC3T3-E1细胞增殖、分化和矿化的有效性,初步筛选频率和强度参数,分别对细胞施加不同参数(频率、强度)的超声,采取CCK-8法检测细胞的增殖情况,碱性磷酸酶检测试剂盒检测分化效果,茜素红染色观察矿化效果。发现30mW/cm²和40mW/cm²的超声对细胞产生促增殖作用,50mW/cm²抑制增殖;1.5MHz和1.7MHz的较低强度组均较对照组的ALP活性有增加;1.5MHz,40mW/cm²的超声组矿化效果较其他组好。研究结果表明,低强度的脉冲超声可以提高成骨细胞株MC3T3-E1增殖、分化和矿化能力,可能是防治骨质疏松的可行手段之一。

The aim of the present study is to investigate the effectiveness of low-intensity pulsed ultrasound(LIPUS) on cell proliferation, differentiation and mineralizationusing of MC3T3-E1 cell line. The MC3T3-E1 cells were exposed to LIPUS with different parameters(frenquency and intensity). The cell proliferations, differentiation, and mineralization were determined by CCK-8 method, alkaline phosphatase detection kit, and alizarin red staining, respectively. The ultrasound intensity of 30mW/cm² and 40mW/cm² could promote cell proliferation, while 50mW/cm² inhibit it. The lower intensity group under 1.5MHz and 1.7MHz present better alkaline phosphatase activity. The 1.5MHz and 40 mW/cm² groups showed better mineralization effect than others. LIPUS can promote MC3T3-E1 on cell proliferation, differentiation and mineralization. LIPUS may act as a new method forprevention and treatment of osteoporosis.