为提高粉红粘帚霉ACM941 发酵过程中的分生孢子产量，本研究对其固体发酵条件进行了优化。以产孢量为测定指标，通过单因子和正交试验，确定ACM941 固体发酵基础培养基为麦麸和棉籽壳混合物，其比例为4：6；在基础培养基中添加2%玉米粉、2%燕麦粉、3%牛肉膏、2%蛋白胨可获得更大产孢量；对发酵温度、接种量等发酵条件进行了优化，确定发酵培养基初始含水量为30%；种龄48 h；接种量6%；发酵过程中24 h 全光照；菌丝生长温度25℃，产孢温度20℃。在最佳发酵条件下，ACM91产孢量达1.17×109 cfu/g 培养物，比初始发酵水平提高了12.4 倍，为生防制剂的开发奠定了基础。
The solid fermentation of Gliocladium roseum ACM941 has not been well studied so far,this paper proposes to improve the fermentation titer of ACM941 through the single factor and orthogonal design. The optimum fermentation conditions are obtained as follows: The bran and cottonseed shell ratio is 4:6, the corn flour 2%, the oat flour 2%, the beef extract 3%, and the peptone 2%. The optimum mycelial growth and sporulation temperatures are 25℃ and 20℃. The initial water content is 30% and the inoculation volume is 6%. Under these conditions, the maximum conidial yield comes to 1.17×109 cfu/g, 12.4 times higher than the initial fermentation level. This provides a support for the development of the biocontrol agent.
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