综述文章

基于代谢组学的头颈癌生物标志物研究进展

  • 张小涛 ,
  • 侯宏卫 ,
  • 刘勇 ,
  • 王安 ,
  • 胡清源
展开
  • 1. 中国科学院合肥物质科学研究院; 中国科学院安徽光学精密机械研究所, 合肥230031;
    2. 国家烟草质量监督检验中心, 郑州450001
张小涛,博士研究生,研究方向为吸烟与健康及生物标志物,电子信箱:zhangxiaotao9209@163.com

收稿日期: 2014-04-01

  修回日期: 2014-04-30

  网络出版日期: 2014-08-01

基金资助

国家自然科学基金项目(21277174)

Biomarkers of Head and Neck Cancers for Metabolomics

  • ZHANG Xiaotao ,
  • HOU Hongwei ,
  • LIU Yong ,
  • WANG An ,
  • HU Qingyuan
Expand
  • 1. Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China;
    2. China National Tobacco Quality Supervision & Test Centre, Zhengzhou 450001, China

Received date: 2014-04-01

  Revised date: 2014-04-30

  Online published: 2014-08-01

摘要

头颈癌被列为世界上第六大癌症,头颈癌的主要诱因是人类乳头瘤病毒、酒精和烟草。由于缺乏早期检测和风险评估的生物标记物,超过50%的头颈癌患者往往在晚期阶段才能确诊,5年期幸存率低于50%。基于代谢组学技术的高通量分析方法具有发现肿瘤相关标志物的潜能,综述了代谢组学技术在头颈癌生物标志物发现中的应用进展和存在的问题,展望了进一步研究的主要方向。

本文引用格式

张小涛 , 侯宏卫 , 刘勇 , 王安 , 胡清源 . 基于代谢组学的头颈癌生物标志物研究进展[J]. 科技导报, 2014 , 32(21) : 66 -71 . DOI: 10.3981/j.issn.1000-7857.2014.21.011

Abstract

The head and neck cancers are the sixth most common cause of cancer deaths. The head and neck squamous cell carcinomas (HNSCCs) are caused by the tobacco and alcohol consumption and by the infection of high-risk types of human papillomavirus. Due to the lack of biomarkers for early detection and risk assessment, more than 50% of head and neck cancer patients are often diagnosed at a late stage, and the five-year survivor rate is lower than 50%. The high-throughput analysis method based on the metabolomics technology has the potential to come up with tumor-associated biomarkers. This paper reviews the progress and challenges of the metabolomics technology in its way into the biomarker discovery of the head and neck cancer, and the future development is also discussed.

参考文献

[1] Schmitz S, Machiels J P. Molecular biology of squamous cell carcinoma of the head and neck relevance and therapeutic implications[J]. Expert Review of Anticancer Therapy, 2010, 10(9): 1471-1484.
[2] Athanassios A, Michalis V K, David R, et al. Head and neck cancer[J]. Lancet, 2008, 371: 1695-1709.
[3] Rezende T M, De Souza Freire M, Franco O L. Head and neck cancer: Proteomic advances and biomarker achievements[J]. Cancer, 2010, 116 (21): 4914-4925.
[4] Schaaij-Visser T B, Brakenhoff R H, Leemans C R, et al. Protein biomarker discovery for head and neck cancer[J]. Journal of Proteomics, 2010, 73(10): 1790-1803.
[5] Parkin D M, Bray F, Ferlay J, et al. Global cancer statistics, 2002[J].CA: A Cancer Journal for Clinicians, 2005, 55(2): 74-108.
[6] Lee K D, Lee H S, Jeon C H. Body fluid biomarkers for early detection of head and neck squamous cell carcinomas[J]. Anticancer Research, 2011, 31(4): 1161-1167.
[7] Atkinson A J, Colburn W A, Degruttola V G, et al. Biomarkers and surrogate endpoints: Preferred definitions and conceptual framework[J]. Clinical Pharmacology & Therapeutics, 2001, 69(3): 89-95.
[8] Stransky N, Egloff A M, Tward A D, et al. The mutational landscape of head and neck squamous cell carcinoma[J]. Science, 2011, 333(6046): 1157-1160.
[9] Agrawal N, Frederick M J, Pickering C R, et al. Exome sequencing of head and neck squamous cell carcinoma reveals inactivating mutations in NOTCH1[J]. Science, 2011, 333(6046): 1154-1157.
[10] Martinez I, Wang J, Hobson K F, et al. Identification of differentially expressed genes in HPV-positive and HPV-negative oropharyngeal squamous cell carcinomas[J]. European Journal of Cancer, 2007, 43 (2): 415-432.
[11] Ralhan R, Masui O, Desouza L V, et al. Identification of proteins secreted by head and neck cancer cell lines using LC-MS/MS: Strategy for discovery of candidate serological biomarkers[J]. Proteomics, 2011, 11(12): 2363-2376.
[12] Ralhan R, DeSouza L V, Matta A, et al. iTRAQ-multidimensional liquid chromatography and tandem mass spectrometry-based identification of potential biomarkers of oral epithelial dysplasia and novel networks between inflammation and premalignancy[J]. Journal of Proteome Research, 2008, 8(1): 300-309.
[13] Ralhan R, DeSouza L V, Matta A, et al. Discovery and verification of head-and-neck cancer biomarkers by differential protein expression analysis using iTRAQ labeling, multidimensional liquid chromatography, and tandem mass spectrometry[J]. Molecular & Cellular Proteomics, 2008, 7(6): 1162-1173.
[14] Chi L M, Lee C W, Chang K P, et al. Enhanced interferon signaling pathway in oral cancer revealed by quantitative proteome analysis of microdissected specimens using 16O/18O labeling and integrated twodimensional LC-ESI-MALDI tandem MS[J]. Molecular & Cellular Proteomics, 2009, 8(7): 1453-1474.
[15] Wu Z, Doondeea J B, Gholami A M, et al. Quantitative chemical proteomics reveals new potential drug targets in head and neck cancer[J]. Molecular & Cellular Proteomics, 2011, 10(12): M111.011635.
[16] Jarai T, Maasz G, Burian A, et al. Mass spectrometry-based salivary proteomics for the discovery of head and neck squamous cell carcinoma[J]. Pathology & Oncology Research, 2012, 18(3): 623-628.
[17] George P. Metabolomics comes of age?[J]. The Scientist, 2005, 19(11): 8.
[18] Raamsdonk L M, Teusink B, Broadhurst D, et al. A functional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations[J]. Nature Biotechnology, 2001, 19(1): 45-50.
[19] Gowda G N, Zhang S C, Gu H W, Asiago V, et al. Metabolomicsbased methods for early disease diagnostics[J]. Expert Review of Molecular Diagnostics, 2008, 8(5): 617-633.
[20] 和红兵, 石先哲, 陈静, 等. 气相色谱-质谱和液相色谱-质谱联用方法用于口腔癌代谢组学分析[J]. 色谱, 2012, 30(3): 245-251. He Hhongbin, Shi Xianzhe, Chen Jing, et al. Metabonomics study of oral cancer using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, 2012, 30(3): 245-251.
[21] Argiris A, Lee S C, Feinstein T, et al. Serum biomarkers as potential predictors of antitumor activity of cetuximab-containing therapy for locally advanced head and neck cancer[J]. Oral Oncology, 2011, 47 (10): 961-966.
[22] Ghannoum M A, Mukherjee P K, Jurevic R J, et al. Metabolomics reveals differential levels of oral metabolites in HIV-infected patients: Toward novel diagnostic targets[J]. Omics: A Journal of Integrative Biology, 2013, 17(1): 5-15.
[23] Wei J, Xie G, Zhou Z, et al. Salivary metabolite signatures of oral cancer and leukoplakia[J]. International Journal of Cancer, 2011, 129 (9): 2207-2217.
[24] Srivastava S, Roy R, Gupta V, et al. Proton HR-MAS MR spectroscopy of oral squamous cell carcinoma tissues: An ex vivo study to identify malignancy induced metabolic fingerprints[J]. Metabolomics, 2010, 7 (2): 278-288.
[25] Somashekar B S, Kamarajan P, Danciu T, et al. Magic angle spinning NMR-based metabolic profiling of head and neck squamous cell carcinoma tissues[J]. Journal of Proteome Research, 2011, 10(11): 5232-5241.
[26] Koh T, Murakami Y, Tanaka S, et al. Changes of metabolic profiles in an oral squamous cell carcinoma cell line induced by eugenol[J]. In Vivo, 2013, 27(2): 233-243.
[27] Sepiashvili L, Hui A, Ignatchenko V, et al. Potentially novel candidate biomarkers for head and neck squamous cell carcinoma identified using an integrated cell line-based discovery strategy[J]. Molecular & Cellular Proteomics, 2012, 11(11): 1404-1415.
[28] Barton R H, Nicholson J K, Elliott P, et al. High-throughput 1H NMR-based metabolic analysis of human serum and urine for largescale epidemiological studies: validation study[J]. International Journal of Epidemiology, 2008, 37(S1): i31-i40.
[29] Bernini P, Bertini I, Luchinat C, et al. Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks[J]. Journal of Biomolecular NMR, 2011, 49(3/4): 231-243.
[30] Zhang A, Sun H, Wu X, et al. Urine metabolomics[J]. Clinica Chimica Acta, 2012, 414: 65-69.
[31] Want E J, Wilson I D, Gika H, et al. Global metabolic profiling procedures for urine using UPLC-MS[J]. Nature Protocols, 2010, 5 (6): 1005-1018.
[32] álvarez-Sánchez B, Priego-Capote F, Castro M D. Metabolomics analysis II. Preparation of biological samples prior to detection[J]. TrAC Trends in Analytical Chemistry, 2010, 29(2): 120-127.
[33] Fernández-Peralbo M A, Luque de Castro M D. Preparation of urine samples prior to targeted or untargeted metabolomics massspectrometry analysis[J]. TrAC Trends in Analytical Chemistry, 2012, 41: 75-85.
[34] Vuckovic D, Pawliszyn J. Systematic evaluation of solid-phase microextraction coatings for untargeted metabolomic profiling of biological fluids by liquid chromatography-mass spectrometry[J]. Analytical Chemistry, 2011, 83(6): 1944-1954.
[35] Vuckovic D. Current trends and challenges in sample preparation for global metabolomics using liquid chromatography-mass spectrometry[J]. Analytical and Bioanalytical Chemistry, 2012, 403(6): 1523-1548.
[36] Monteiro M S, Carvalho M, Bastos M L, et al. Metabolomics analysis for biomarker discovery: advances and challenges[J]. Current Medicinal Chemistry, 2013, 20(2): 257-271.
[37] López-Gresa M P, Maltese F, Bellés J M, et al. Metabolic response of tomato leaves upon different plant-pathogen interactions[J]. Phytochemical Analysis, 2010, 21(1): 89-94.
[38] Wang X, Sun H, Zhang A, et al. Ultra-performance liquid chromatography coupled to mass spectrometry as a sensitive and powerful technology for metabolomic studies[J]. Journal of Separation Science, 2011, 34(24): 3451-3459.
[39] Spratlin J L, Serkova N J, Eckhardt S G. Clinical applications of metabolomics in oncology: A review[J]. Clinical Cancer Research, 2009, 15(2): 431-440.
[40] Somberg J C. Biomarker Methods in drug discovery and development[J]. American Journal of Therapeutics, 2009, 16(5): 466-467.
[41] Wang J H, Byun J, Pennathur S. Analytical approaches to metabolomics and applications to systems biology[J]. Seminars in Nephrology, 2010, 30(5): 500-511.
[42] Putri S P, Yamamoto S, Tsugawa H, et al. Current metabolomics: Technological advances[J]. Journal of Bioscience and Bioengineering, 2013, 116(1): 9-16.
[43] Xiao J F, Zhou B, Ressom H W. Metabolite identification and quantitation in LC-MS/MS-based metabolomics[J]. Trends in Analytical Chemistry: TRAC, 2012, 32:1-14.
[44] Spagou K, Wilson I D, Theodoridis G. HILIC-UPLC-MS for exploratory urinary metabolic profiling in toxicological studies[J]. Analytical Chemistry, 2011, 83(1): 382-390.
[45] Spagou K, Tsoukali H, Raikos N, et al. Hydrophilic interaction chromatography coupled to MS for metabonomic/metabolomic studies[J]. Journal of Separation Science, 2010, 33(6/7): 716-727.
[46] Büscher J M, Czernik D, Ewald J C, et al. Cross-platform comparison of methods for quantitative metabolomics of primary metabolism[J]. Analytical Chemistry, 2009, 81(6): 2135-2143.
[47] 许国旺. 代谢组学:方法与应用[M]. 北京: 科学出版社, 2008. Xu Guowang, Metabonomics: Methods and application[M]. Beijing: Science Press, 2008.
[48] Yan S K, Wei B J, Lin Z Y, et al. A metabonomic approach to the diagnosis of oral squamous cell carcinoma, oral lichen planus and oral leukoplakia[J]. Oral Oncology, 2008, 44(5): 477-483.
[49] 李丹娟. 鼻咽癌血清代谢组学初步研究[D]. 长沙: 中南大学, 2009. Li Danjuan. Research on serum metabonomics of the nasopharyngeal carcinoma[D]. Changsha: Central South University, 2009.
[50] Yao Z, Yin P, Su D, et al. Serum metabolic profiling and features of papillary thyroid carcinoma and nodular goiter[J]. Molecular Biosystems, 2011, 7(9): 2608-2614.
[51] Tang F, Xie C, Huang D, et al. Novel potential markers of nasopharyngeal carcinoma for diagnosis and therapy[J]. Clinical Biochemistry, 2011, 44(8/9): 711-718.
[52] Leng J, Guan Q, Sun T, et al. Application of isotope-based carboxy group derivatization in LC-MS/MS analysis of tissue free-fatty acids for thyroid carcinoma[J]. Journal of Pharmaceutical and Biomedical Analysis, 2013, 84: 256-262.
文章导航

/