为探讨不同剂量异常黑胆质成熟剂对异常黑胆质载体大鼠心肌缺血再灌注损伤的影响，将72 只异常黑胆质模型大鼠和24 只健康雄性SD 大鼠随机分为正常假手术组模型假手术组正常缺血再灌注组模型缺血再灌注组成熟剂干预组（低剂量中剂量及高剂量组）阿托伐他汀干预组8 组各12 只。采用酶联免疫法（ELISA）检测各组大鼠血清心肌酶及肌钙蛋白的水平；苏木精—伊红（HE）染色及高倍电镜方法观察不同组别大鼠心肌组织及超微结构的变化。成熟剂干预组大鼠心肌酶及肌钙蛋白水平明显低于模型缺血再灌注组，其中中剂量组最低；HE 染色心肌组织变化显示，成熟剂干预组大鼠心肌水肿肌纤维增殖较模型组明显；中剂量组大鼠心肌组织损伤最轻。中到高剂量的异常黒胆质成熟剂对缺血再灌注损伤的保护作用可能优于阿托伐他汀，但尚需要进一步的研究来证实。
This paper aims to investigate the effects of different doses of abnormal Savda Munziq on myocardial ischemia-reperfusion injury (MI/RI) in rats with the abnormal Savda syndrome. Seventy-two rats with abnormal Savda syndrome and 24 healthy ones were selected and divided into eight groups: ischemia-reperfusion model group, high dose maturation agent group, middle dose group mature agent, small doses of maturation agent group, normal ischemia-reperfusion group, model sham group and atorvastatin intervention group (positive control group). Each group contained 12 rats. The enzyme-linked immunosorbent assay (ELISA) was used to detect the serum myocardial enzyme and troponin levels, and hematoxylin and eosin (HE) staining was used to observe changes of the myocardial tissues in the different groups. The results showed that in the Munziq intervention groups, the serum creatine kinase and troponin levels were significantly lower than those in the model group, and the middle-dose group showed the lowest levels. The HE staining of myocardial tissue showed that the myocardial edema and muscle fiber proliferation levels were significantly higher in the Munziq intervention groups than in the model ischemia-reperfusion injury group, and the middle-dose group showed the least cardiac tissue damage. Moderate to high doses of abnormal Savda Munziq may be more effective on abnormal Savda ischemia-reperfusion injury in rats than atorvastatin, but further studies are still needed to confirm the conclusion.
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