为探讨维药异常黑胆质成熟剂对乳腺增生模型大鼠的治疗作用及可能的作用机制，选用清洁级雌性Wistar 大鼠60 只，随机分为6 组：正常对照组、模型组、阳性对照组（三苯氧胺组）、异常黑胆质成熟剂高、中、低剂量组，采用大鼠右下肢肌肉注射雌、孕激素建立乳腺增生病模型，进行药物干预（三苯氧胺1.8 mg/kg，胆质成熟剂高、中、低剂量分别为3.75、7.5、15 g/kg）。运用光学显微镜观察维药异常黑胆质成熟剂对实验性乳腺增生症大鼠乳腺形态学的影响，放射免疫测定法检测其血清激素水平。结果表明，异常黑胆质成熟剂能减轻上皮细胞、间质纤维组织增生程度，改善模型大鼠乳腺组织病理形态学改变，调节血清激素水平，降低血清雌二醇（E2）、黄体生成素（LH）、升高孕酮（P）、睾酮（T）、催乳素（PRL）及卵泡刺激素（FSH）水平，与模型组比较具有统计学差异（P<0.01 或P<0.05）。由此可见，异常黑胆质成熟剂对乳腺增生症大鼠的乳腺组织形态改变有一定的修复作用，同时能够降低大鼠血清雌激素水平，显著提高孕激素水平，且雌、孕激素比例失调状态也能够得到改善。
This paper discusses the therapeutic effect and its possible mechanismof abnormal Savda Munziq (ASMq) on galactophore hyperplasia (GH) in rats. Sixty rats were randomly divided into six groups, namely the control group, model group,positive control (tamoxifen) group, ASMq high-dose group, ASMq middle-dose group, and ASMq low-dose group. The rat pathologic model of GH was established by estradiol/progesterone injection and drug intervention (tamoxifen 1.8 mg/kg; ASMq high, middle and low dose: 15, 7.5, 3.75 mg/kg). The effect of ASMq on the morphological structure of breast tissue in the GH rat model was studied using an optical microscope and the concentration of sex hormones in serum was observed by radio immunoassay. The result indicates that ASMq can reduce the degree of hyperplasia of the epithelial cells and fibrous tissue, improve the pathological changes of breast tissue of model rats, regulate the serum hormone level, decrease the concentration of estradiol (E2), luteinizing hormone (LH), and elevate the level of progesterone (P), testosterone (T), prolactin (PRL) and follicle stimulating hormone (FSH). Significant differences were found compared with the model group (P<0.01 or P<0.05). Therefore, we conclude that ASMq can recover the morphological damage of breast tissue in the GH model, and at the same time, it can reduce the serum E2 level and increase serum P level, improving the state of imbalance between E2 and P.
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